- 询价
- Cell Signaling Technology
- 3014
- USA
- 2025年12月19日
- IHC-P, IF-F, IF-IC, F
- H,M,R
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- 详细信息
- 文献和实验
- 技术资料
- 抗体英文名：
Insulin (C27C9) Rabbit mAb
- 抗原：
synthetic peptide corresponding to the sequence of human insulin
- 应用范围：
IHC-P, IF-F, IF-IC, F
- 供应商：
CST
- 保质期：
详见说明书
- 适应物种：
H,M,R
- 库存：
大量
- 级别：
详见MSDS文件
- 是否单克隆：
1
- 保存条件：
-20°c
- 规格：
100 ul/carrier free & custom formulation / quantity
| 规格： | 产品价格： | ￥请询价 | |
|---|---|---|---|
| 规格： | 100 ul | 产品价格： | ￥请询价 |
| 规格： | carrier free & custom formulation / quantity | 产品价格： | ￥请询价 |
pathway more info application references datasheet PDF MSDS PDF protocols
Applications Key: IHC-P=Immunohistochemistry (Paraffin) IF-F=Immunofluorescence (Frozen) IF-IC=Immunofluorescence (Immunocytochemistry) F=Flow Cytometry
Reactivity Key: H=Human M=Mouse R=Rat
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.
| Applications | Reactivity | Sensitivity | MW (kDa) | Isotype |
|---|---|---|---|---|
| IHC-P IF-F IF-IC F | H M R | Endogenous | 6 | Rabbit IgG |
| Protocols |
|
|---|---|
| Specificity / Sensitivity | Insulin (C27C9) Rabbit mAb detects endogenous levels of total insulin protein. |
| Source / Purification | Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to the sequence of human insulin. IHC-P (paraffin)
Immunohistochemical analysis of paraffin-embedded human pancreas, showing the staining of β cells, using Insulin (C27C9) Rabbit mAb. IHC-P (paraffin)
Immunohistochemical anaylsis of paraffin-embedded mouse pancreas, showing staining of β cells, using Insulin (C27C9) Rabbit mAb. IHC-P (paraffin)
Immunohistochemical analysis of paraffin-embedded rat pancreas, showing staining of β cells, using Insulin (C27C9) Rabbit mAb. Flow Cytometry
Flow cytometric analysis of HeLa cells (blue) and β-TC-6 cells (green) using Insulin (C27C9) Rabbit mAb. IF-IC
Confocal immunofluorescent analysis of β-TC-6 (left) and C2C12 cells (right) using Insulin (C27C9) Rabbit mAb (green). Actin filaments have been labeled with DY-554 phalloidin (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye). IF-F
Confocal immunofluorescent analysis of rat pancreas using Insulin (C27C9) Rabbit mAb (green). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye). |
| Background | The maintenance of glucose homeostasis is an essential physiological process that is regulated by hormones. An elevation in blood glucose levels during feeding stimulates insulin release from pancreatic β cells through a glucose sensing pathway (1). Insulin is synthesized as a precursor molecule, proinsulin, which is processed prior to secretion. A- and B-peptides are joined together by a disulfide bond to form insulin, while the central portion of the precursor molecule is cleaved and released as the C-peptide. Insulin stimulates glucose uptake from blood into skeletal muscle and adipose tissue. Insulin deficiency leads to type 1 diabetes mellitus (2). |
| Application References | Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know ! |
| Companion Products |
For Research Use Only. Not For Use In Diagnostic Procedures. |
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文献和实验Although the rabbit is used extensively in developmental toxicity testing, relatively little is known about the fundamental developmental biology of this species let alone mechanisms underlying developmental toxicity. This paucity
Purification of mAb (IgG) by Chang-Duk Jun, 03/14/2000 Purpose Materials Antibody 7E3 , 2L sup grown in flasks, frozen and thawed overnight. BioRad Affi-Gel Protein A MAPS II Buffers
胰岛素 insulin 脊椎动物胰腺中兰氏岛（ Yangerhans）的β细胞分泌的激素。 1921年由 F.G.Banting和 C.H.Best所发现。 insulin一名系由 insula（岛）而来。胰岛素可用酸性乙醇从胰腺中提取。 1926年 J.J.Abel已分离出胰岛素结晶，结晶中含有微量锌。单体的分子量为 5700，在中性溶液中可互相融合。 F.Sanger就作为牛胰岛素的蛋白质曾首次确定了其氨基酸的排列顺序（ 1955）。胰岛素的结构是，通过 S— S键
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