产品封面图

Bcl-xL (54H6) Rabbit mAb

收藏
  • 询价
  • Cell Signaling Technology已认证
  • USA
  • 2025年07月18日
  • western blot,免疫沉淀（IP）,免疫组化（IHC）,免疫组化（IHC）,免疫荧光（IF）,流式细胞（Flow Cyt）
  • 兔
  • 人,小鼠,大鼠,驴
avatar
美国CST中国公司(丁香通商铺)
品牌商
13 年钻石会员

  • 企业认证

    • 相关产品推荐更多 >

    万千商家帮你免费找货

    0 人在求购买到急需产品
    • 详细信息
    • 询价记录
    • 文献和实验
    • 技术资料
    • 抗体英文名：

      Bcl-xL (54H6) Rabbit mAb

    • 抗原：

      /

    • 应用范围：

      western blot,免疫沉淀（IP）,免疫组化（IHC）,免疫组化（IHC）,免疫荧光（IF）,流式细胞（Flow Cyt）

    • 宿主：

      兔

    • 级别：

      详见MSDS文件

    • 库存：

      大量

    • 抗原来源：

      /

    • 保质期：

      详见说明书

    • 供应商：

      CST

    • 适应物种：

      人,小鼠,大鼠,驴

    • 是否单克隆：

      1

    • 保存条件：

      -20°c

    • 规格：

      40 ul (4 western blots)/100 ul (10 western blots)/<a href="http://www.cellsignal.com/ddt/custom_reagents.html" target="_blank">carrier free &amp; custom formulation / quantity</a> /40 ul (4 western blots)/100 ul (10 western blots)/<a href="http://www.cellsignal.com/ddt/custom_reagents.html" target="_blank">carrier free &amp; custom formulation / quantity</a>

    规格：产品价格：￥面议
    规格：40 ul (4 western blots)产品价格：￥请询价
    规格：100 ul (10 western blots)产品价格：￥请询价
    规格：<a href="http://www.cellsignal.com/ddt/custom_reagents.html" target="_blank">carrier free &amp; custom formulation / quantity</a> 产品价格：￥请询价
    规格：40 ul (4 western blots)产品价格：￥请询价
    规格：100 ul (10 western blots)产品价格：￥请询价
    规格：<a href="http://www.cellsignal.com/ddt/custom_reagents.html" target="_blank">carrier free &amp; custom formulation / quantity</a> 产品价格：￥请询价

    Product Pathways - Apoptosis

    Bcl-xL (54H6) Rabbit mAb #2764

    Applications Reactivity Sensitivity MW (kDa) Isotype
    W IP IHC-P IHC-F IF-IC F H M R Mk Endogenous 30 Rabbit IgG

    Applications Key:  W=Western Blotting  IP=Immunoprecipitation  IHC-P=Immunohistochemistry (Paraffin)  IHC-F=Immunohistochemistry (Frozen)  IF-IC=Immunofluorescence (Immunocytochemistry)  F=Flow Cytometry
    Reactivity Key:  H=Human  M=Mouse  R=Rat  Mk=Monkey
    Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

    Protocols

    * Product-specific protocol.

    Specificity / Sensitivity

    Bcl-xL (54H6) Rabbit mAb detects endogenous levels of total Bcl-xL protein. The antibody does not cross-react with other Bcl-2 family members.

    Source / Purification

    Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Asp61 of human Bcl-xL.

    Western Blotting

    Western Blotting

    Western blot analysis of extracts from Jurkat and HeLa (human), COS (monkey), NIH/3T3 and L929 (mouse), and PC12 and C6 (rat) cells, using Bcl-xL (54H6) Rabbit mAb.

    Western Blotting

    Western Blotting

    Western blot analysis of extracts from HeLa cells, transfected with 100 nM SignalSilence® Control siRNA (Unconjugated) #6568 (-), SignalSilence® BcL-xL siRNA I (+) or SignalSilence® Bcl-xL siRNA II #6363 (+), using Bcl-xL (54H6) Rabbit mAb #2764 (upper) or α-Tubulin (11H10) Rabbit mAb #2125 (lower). The Bcl-xL (54H6) Rabbit mAb confirms silencing of Bcl-xL expression, while the α-Tubulin (11H10) Rabbit mAb is used as a loading control.

    IP

    IP

    Immunoprecipitation of Bcl-xL from Jurkat cell extracts, using Bcl-xL (54H6) Rabbit mAb. Lane 1 is the lysate control, lane 2 is antibody alone and lane 3 is antibody plus lysate.


    IHC-P (paraffin)

    IHC-P (paraffin)

    Immunohistochemical analysis of paraffin-embedded human lung carcinoma, using Bcl-xL (54H6) Rabbit mAb.

    IHC-P (paraffin)

    IHC-P (paraffin)

    Immunohistochemical analysis of paraffin-embedded 4TI syngeneic mouse tumor, using Bcl-xL (54H6) Rabbit mAb # 2764.

    IHC-P (paraffin)

    IHC-P (paraffin)

    Immunohistochemical analysis of paraffin-embedded human colon carcinoma, using Bcl-xL (54H6) Rabbit mAb in the presence of control peptide (left) or Bcl-xL Blocking Peptide #1225 (right).


    IHC-P (paraffin)

    IHC-P (paraffin)

    Immunohistochemical analysis of paraffin-embedded human prostate carcinoma, showing cytoplasmic localization, using Bcl-xL (54H6) Rabbit mAb.

    IHC-F (frozen)

    IHC-F (frozen)

    Immunohistochemical analysis of frozen H1650 xenograft, showing cytoplasmic localization using Bcl-xL (54H6) Rabbit mAb.

    Flow Cytometry

    Flow Cytometry

    Flow cytometric analysis of untreated Jurkat cells, using Bcl-xL (54H6) Rabbit mAb (blue) compared to a nonspecific negative control antibody (red).


    IF-IC

    IF-IC

    Confocal immunofluorescent analysis of HeLa cells using Bcl-xL (54H6) Rabbit mAb (green). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).

    Background

    Bcl-xL prevents apoptosis through two different mechanisms: heterodimerization with an apoptotic protein inhibits its apoptotic effect (1,2) and formation of mitochondrial outer membrane pores help maintain a normal membrane state under stressful conditions (3). Bcl-xL is phosphorylated by JNK following treatment with microtubule-damaging agents such as paclitaxel, vinblastine and nocodazole (4,5).

    1. Adams, J.M. and Cory, S. (1998) Science 281, 1322-1326.
    2. Minn, A.J. et al. (1999) EMBO. J. 18, 632-643.
    3. Vander Heiden, M.G. et al. (2001) J. Biol. Chem. 276, 19414-19419.
    4. Fan, M. et al. (2000) J. Biol. Chem. 275, 29980-29985.
    5. Poruchynsky, M.S. et al. (1998) Cancer Res. 58, 3331-3338.

    Application References

    Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know !

    Companion Products

    Rabbit Monoclonals Produced Using Epitomics® Technology, U.S. Patent No. 5,675,063.

    For Research Use Only. Not For Use In Diagnostic Procedures.

    风险提示：丁香通仅作为第三方平台，为商家信息发布提供平台空间。用户咨询产品时请注意保护个人信息及财产安全，合理判断，谨慎选购商品，商家和用户对交易行为负责。对于医疗器械类产品，请先查证核实企业经营资质和医疗器械产品注册证情况。

    • 作者
    • 内容
    • 询问日期
    图标文献和实验
    相关实验

    • 组化染色背景高？没信号？一篇文章带你快速掌握免疫组化！

      ：使用 Anti-phospho-Akt (Ser473) Rabbit mAb 对石蜡包埋的人乳腺癌组织进行免疫组织化学分析。（图 A）使用免疫组化试剂盒M&R HRP/DAB Detection IHC Kit，抗体 1:100 稀释；(图 B) 采用普通免疫组化试剂盒，抗体 1:25 稀释。 图 6 免疫组化实验检测 Erk1/2 表达 注：使用 Anti-Erk1/2 Mouse mAb与p44/42 MAPK （Erk1/2）Rabbit mAb 对正常小鼠心脏组织进行免疫

    • 三生三世，一场组化，一次豪赌

      h）即可。2. 修复大法——不仅仅是「煮一煮」微波炉修复：简单易行效果好，CST 推荐使用微波炉完成修复。合适的修复液：根据抗体说明书使用合适的修复液。用柠檬酸修复后，切片需浸泡在修复液中，自然冷却；而用 EDTA 修复后，切片可直接从修复缸中取出，直接进行下一步。注：使用不同的修复方式和不同生产商的抗体检测人肺癌组织中 EGFR 的表达。第一排为 CST 的 EGF Receptor (D38B1) XP® Rabbit mAb（#4267），EDTA 的修复方式明显优于柠檬酸盐及胃蛋白

    • 如何做出一手漂亮的 WB 结果？

      到 PVDF 膜上，同时减少蛋白不必要降解，这对于最终获得清晰、可信结果也是需要考虑因素。抗体信息：1.ACC1, Recombinant Rabbit monoclonal IgG. HuaAn. HuaAn biotechnology , inc.2.ATGL, Mouse mAb IgG1, Cat#:RT1058. HuaAn biotechnology , inc.3.p-PERK(Thr981), Rabbit Polyclonal IgG primary antibodies, Cat

    查看更多相关实验 >
    图标技术资料

    暂无技术资料 索取技术资料

    同类产品报价

    产品名称
    产品价格
    公司名称
    报价日期
    Bcl-xL (54H6) Rabbit mAb
    询价
    上海谷研实业有限公司
    2026年01月19日询价
    Bcl-xL (54H6) Rabbit mAb
    ￥1632
    上海瑶韵生物科技有限公司
    2025年07月14日询价
    Bcl-xL (54H6) Rabbit mAb
    ￥4043
    上海岑特生物科技有限公司
    2025年06月30日询价
    Bcl-xL (54H6) Rabbit mAb
    ￥1632
    北京智杰方远科技有限公司
    2023年10月25日询价
    Bcl-xL (54H6) Rabbit mAb
    ￥3850
    杭州囊萤科技有限公司
    2025年05月20日询价
    Bcl-xL (54H6) Rabbit mAb
    询价