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- Cell Signaling Technology
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- 2025年09月07日
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- 详细信息
- 文献和实验
- 技术资料
- 抗体英文名：
EGF Receptor (1F4) Mouse mAb
- 抗原：
recombinant protein corresponding to the C-terminal sequence of human EGF receptor
- 应用范围：
W
- 供应商：
CST
- 适应物种：
H
- 库存：
大量
- 保质期：
详见说明书
- 级别：
详见MSDS文件
- 是否单克隆：
1
- 保存条件：
-20°c
- 规格：
100 ul (10 western blots)/carrier free & custom formulation / quantity
| 规格： | 产品价格： | ￥请询价 | |
|---|---|---|---|
| 规格： | 100 ul (10 western blots) | 产品价格： | ￥请询价 |
| 规格： | carrier free & custom formulation / quantity | 产品价格： | ￥请询价 |
pathway more info application references datasheet PDF MSDS PDF protocols
Applications Key: W=Western Blotting
Reactivity Key: H=Human
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.
| Applications | Reactivity | Sensitivity | MW (kDa) | Isotype |
|---|---|---|---|---|
| W | H | Endogenous | 175 | Mouse IgG1 |
| Protocols |
|
|---|---|
| Specificity / Sensitivity | EGF Receptor(1F4) Mouse mAb detects endogenous levels of EGF receptors. It does not cross-react with other Erb family members. |
| Source / Purification | Monoclonal antibody is produced by immunizing animals with a recombinant protein corresponding to the C-terminal sequence of human EGF receptor. Western Blotting
Western blot analysis of extracts from the indicated cell lines. EGF Receptor(1F4) Mouse mAb specifically detects EGF receptors expressed in A431 and MDA-MD-468 cells, but does not cross-react with ErbB2, 3, and 4 proteins expressed in MDA-MD-453 and MCF-7 cells. |
| Background | The epidermal growth factor (EGF) receptor is a transmembrane tyrosine kinase that belongs to the HER/ErbB protein family. Ligand binding results in receptor dimerization, autophosphorylation, activation of downstream signaling, internalization, and lysosomal degradation (1,2). Phosphorylation of EGF receptor (EGFR) at Tyr845 in the kinase domain is implicated in stabilizing the activation loop, maintaining the active state enzyme, and providing a binding surface for substrate proteins (3,4). c-Src is involved in phosphorylation of EGFR at Tyr845 (5). The SH2 domain of PLCγ binds at phospho-Tyr992, resulting in activation of PLCγ-mediated downstream signaling (6). Phosphorylation of EGFR at Tyr1045 creates a major docking site for the adaptor protein c-Cbl, leading to receptor ubiquitination and degradation following EGFR activation (7,8). The GRB2 adaptor protein binds activated EGFR at phospho-Tyr1068 (9). A pair of phosphorylated EGFR residues (Tyr1148 and Tyr1173) provide a docking site for the Shc scaffold protein, with both sites involved in MAP kinase signaling activation (2). Phosphorylation of EGFR at specific serine and threonine residues attenuates EGFR kinase activity. EGFR carboxy-terminal residues Ser1046 and Ser1047 are phosphorylated by CaM kinase II; mutation of either of these serines results in upregulated EGFR tyrosine autophosphorylation (10).
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| Application References | Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know ! |
| Companion Products |
For Research Use Only. Not For Use In Diagnostic Procedures. |
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文献和实验A Historical Perspective of the EGF Receptor and Related Systems
Since the isolation of epidermal growth factor (EGF) from mouse submaxillary glands in the early 1950s by Cohen and coworkers, this growth factor has been shown to have various effects on numerous cellular systems. The biological
Mouse Strains and Genetic Nomenclature
., Dlugosz, A.A., Hansen, L.A., Tennenbaum, T., Lichti, U., Yee, D., La Mantia, C., Mourton, T., Herrup, K., and Harris, R.C. 1995. Targeted disruption of mouse EGF receptor: Effect of genetic background on mutant phenotype. Science 269:230‐234
【翻译】Development trends for monoclonal antibody cancer therapeutics
7 and patients frequently developed antibodies to the mouse-derived proteins, which limited their utility8, 9. In addition, only certain murine mAb isotypes have been shown to effectively bind to and activate elements of the human immune
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