- 询价
- Cell Signaling Technology
- 5473
- USA
- 2025年10月26日
- W
- Rabbit
- H,R,Mk
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- 详细信息
- 文献和实验
- 技术资料
- 抗体英文名：
Phospho-Src (Ser17) Antibody
- 抗原：
synthetic peptide corresponding to residues surrounding Ser17 of human Src protein
- 应用范围：
W
- 宿主：
Rabbit
- 库存：
大量
- 供应商：
CST
- 适应物种：
H,R,Mk
- 级别：
详见MSDS文件
- 保质期：
详见说明书
- 是否单克隆：
2
- 保存条件：
-20°c
- 规格：
100 ul (10 western blots)/carrier free & custom formulation / quantity
| 规格： | 产品价格： | ￥请询价 | |
|---|---|---|---|
| 规格： | 100 ul (10 western blots) | 产品价格： | ￥请询价 |
| 规格： | carrier free & custom formulation / quantity | 产品价格： | ￥请询价 |
pathway more info application references datasheet PDF MSDS PDF protocols
Applications Key: W=Western Blotting
Reactivity Key: H=Human R=Rat Mk=Monkey
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.
| Applications | Reactivity | Sensitivity | MW (kDa) | Source |
|---|---|---|---|---|
| W | H R Mk | Endogenous | 60 | Rabbit |
| Protocols |
|
|---|---|
| Specificity / Sensitivity | Phospho-Src (Ser17) Antibody recognizes endogenous levels of Src protein only when phosphorylated at Ser17. |
| Source / Purification | Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ser17 of human Src protein. Antibodies are purified by protein A and peptide affinity chromatography. Western Blotting
Western blot analysis of extracts from C2C12 and MCF7 cells, untreated (-) or treated with λ phosphatase (+), using Phospho-Src (Ser17) Antibody or Src (32G5) Rabbit mAb #2123. |
| Background | The Src family of protein tyrosine kinases, which includes Src, Lyn, Fyn, Yes, Lck, Blk, and Hck, are important in the regulation of growth and differentiation of eukaryotic cells (1). Src activity is regulated by tyrosine phosphorylation at two sites, but with opposing effects. While phosphorylation at Tyr416 in the activation loop of the kinase domain upregulates enzyme activity, phosphorylation at Tyr527 in the carboxy-terminal tail by Csk renders the enzyme less active (2). Protein kinase A (PKA)-dependent phosphorylation of Src at Ser17 is thought to influence multiple signaling networks (3-5). |
| Application References | Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know ! |
| Companion Products |
For Research Use Only. Not For Use In Diagnostic Procedures. |
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文献和实验Analysis of Myristoylated and Palmitoylated Src Family Proteins
acid attachment to the polypeptide backbone. Most proteins destined to become N-myrrstoylated contain the sequence: Met-Gly-X-X-X-Ser/Thr at their N-terminn. After the initrating methionine is removed, the 14-carbon fatty acid myristate is attached
Using Phospho‐Motif Antibodies to Determine Kinase Substrates
comprising both the phosphorylated residue and the surrounding residues that determine kinase specificity, with degenerate residues taking up the remaining positions. Currently, several categories of phospho?motif antibody are commercially available
Optimized Protocol to Make Phospho-Specific Antibodies that Work
, not simply its level of expression. In this review, we will discuss both the design of the phosphopeptide immunogen and immunization. The affinity purification of the phospho-specific antibody as well as the methods most suitable for characterizing
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