- ￥5600
- GeneTex
- 美国
- GTX80395
- 2025年07月14日
- WB, ICC/IF, IHC-P, FACS, ELISA
- Mouse
- Human, Mouse, Rat, Monkey
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- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
Purified recombinant fragment of human CARM1 expressed in E. Coli.
- 亚型：
IgG1
- 形态：
Liquid
- 保存条件：
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
- 克隆性：
Monoclonal
- 标记物：
Unconjugated
- 适应物种：
Human, Mouse, Rat, Monkey
- 保质期：
12 months from the shipping date of the product.
- 抗原来源：
Human
- 目录编号：
GTX80395
- 级别：
Primary Antibodies
- 库存：
Available
- 供应商：
GeneTex
- 宿主：
Mouse
- 应用范围：
WB, ICC/IF, IHC-P, FACS, ELISA
- 靶点：
CARM1
- 抗体英文名：
CARM1 antibody [3H2]
- 抗体名：
CARM1 抗体 [3H2]
- 规格：
100 μl
FACS analysis of Lovo cells using GTX80395 CARM1 antibody [3H2].
Green : CARM1
Purple : negative control
WB analysis of MCF-7 (1), HeLa (2), NIH3T3 (3), HL-60 (4), LNcap (5), Jurkat (6), PC-3 (7), Cos7 (8), and PC-12 (9) cell lysate using GTX80395 CARM1 antibody [3H2].
ICC/IF analysis of HeLa cells using GTX80395 CARM1 antibody [3H2].
Green : CARM1
Red: Actin filaments
IHC-P analysis of breast cancer tissue (left) and ovarian cancer tissue (right) using GTX80395 CARM1 antibody [3H2].
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文献和实验3, mouse CARM1 (PRMT4), human JBP1 (PRMT5). Substrates of PRMTs: In mammalian cells hnRNPs contain about 65% of the total NG,NG-dimethylarginine found in the cell nucleus. Additionally, functionally diverse proteins are substrates for PRMT family
b-Gal Staining of Murine Embryos
Solutions 500mM K4Fe(CN)6.3H2O (Potassium Ferrocyanide) (Sigma: P9387) in DDW 500mM K3Fe(CN)6 (Potassium Ferricyanide) in DDW Solution A : 0.2M NaH2PO4.H2O (27.6g/l) Solution B : 0.2M Na2HPO4 (28,4g/l) Phosphate buffer : 23ml of 0.2M NaH2
SDS-PAGE，蛋白转印，western Blot试剂配制
DTT (20 ml)1．称取3.09 g DTT，加入到50 ml塑料离心管中。2．加20 ml的0.01 M NaOAc (pH5.2)，溶解后使用0.22μm滤器过滤除菌。3．分装后－20oC保存。（参照《分子克隆》二版P884） 3 M 醋酸钠（NaOAc）（100 ml）1．称取40.8 g NaOAc•3H2O置于100～200ml烧杯中，加入约40ml的去离子水后搅拌溶解。2．加冰醋酸调节pH值至5.2。3．定容至100 ml，高压灭菌后室温保存。（参照《分子克隆》二版P884
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