- ￥4300
- GeneTex
- 美国
- GTX70243
- 2025年07月13日
- WB, ICC/IF, IP
- Mouse
- Human, Mouse
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- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
Full-length human RPA-32 expressed in E. coli.
- 亚型：
IgG2a
- 形态：
Liquid
- 保存条件：
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
- 克隆性：
Monoclonal
- 标记物：
Unconjugated
- 适应物种：
Human, Mouse
- 保质期：
12 months from the shipping date of the product.
- 抗原来源：
Human
- 目录编号：
GTX70243
- 级别：
Primary Antibodies
- 库存：
Available
- 供应商：
GeneTex
- 宿主：
Mouse
- 应用范围：
WB, ICC/IF, IP
- 浓度：
1 mg/ml (Please refer to the vial label for the specific concentration.)
- 靶点：
RPA32
- 抗体英文名：
RPA32 antibody [12F3.3]
- 抗体名：
RPA32 抗体 [12F3.3]
- 规格：
100 μl
RPA32 antibody [12F3.3] detects RPA32 protein at DNA damage foci by immunofluorescent analysis.
Sample: 2mM hydroxyurea treated (right) or untreated (left) HeLa cells were fixed in ice-cold MeOH for 5 min.
Green: RPA32 protein stained by RPA32 antibody [12F3.3] (GTX70243) diluted at 1:1000.
Blue: Hoechst 33342 staining.
Scale bar = 10 μm.
Detection of human RPA32 protein using GeneTex RPA32 12F3.3 monoclonal antibody (GTX70243) in HeLa (IR treated) whole cell extract. No radiation, 1hr, 2hr, and 4hr exposure corresponding to lanes 1 through 4 respectively.
RPA32 antibody detects RPA32 protein by western blot analysis. Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and blotted with RPA32 antibody (GTX70243) diluted by 1:500. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.
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文献和实验Yoshihara T et al., EMBO J 2004 (PMID:14749735)
Beck C et al., Nucleic Acids Res 2014 (PMID:24598253)
in detail here. The first protocol is essentially a dual-pulldown assay employing Flag-tagged DNMT3A or antibody of choice for an endogenous protein and 5' biotin linked antisense RNA (Figure 1a), while the second protocol is a triple pulldown assay which essentially expands
上海西唐生物科技有限公司 021-55229872, 65333639 www.westang.com 大鼠白介素 -32(IL-32)ELISA 试剂盒 ( 用于血清、血浆、细胞培养上清液和其它生物体液内 ) 原理 本实验采用双抗体夹心 ABC-ELISA 法。用抗大鼠 IL-32 单抗包被于酶标板上，标准品和样品中的 IL-32 与单抗结合，加入生物素化的抗大鼠 IL-32 ，形成免疫复合物连接
上海西唐生物科技有限公司 021-55229872, 65333639 www.westang.com 人白介素-32 (IL-32)ELISA 试剂盒 ( 用于血清、血浆、细胞培养上清液和其它生物体液内 ) 原理 本实验采用双抗体夹心 ABC-ELISA 法。用抗人 IL-32 单抗包被于酶标板上，标准品和样品中的 IL-32与单抗结合，加入生物素化的抗人 IL
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