Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
克隆性:
Monoclonal
标记物:
Unconjugated
适应物种:
Human, Mouse
保质期:
12 months from the shipping date of the product.
抗原来源:
Human
目录编号:
GTX70243
级别:
Primary Antibodies
库存:
Available
供应商:
GeneTex
宿主:
Mouse
应用范围:
WB, ICC/IF, IP
浓度:
1 mg/ml (Please refer to the vial label for the specific concentration.)
靶点:
RPA32
抗体英文名:
RPA32 antibody [12F3.3]
抗体名:
RPA32 抗体 [12F3.3]
规格:
100 μl
RPA32 antibody [12F3.3] detects RPA32 protein at DNA damage foci by immunofluorescent analysis. Sample: 2mM hydroxyurea treated (right) or untreated (left) HeLa cells were fixed in ice-cold MeOH for 5 min. Green: RPA32 protein stained by RPA32 antibody [12F3.3] (GTX70243) diluted at 1:1000. Blue: Hoechst 33342 staining. Scale bar = 10 μm.
Detection of human RPA32 protein using GeneTex RPA32 12F3.3 monoclonal antibody (GTX70243) in HeLa (IR treated) whole cell extract. No radiation, 1hr, 2hr, and 4hr exposure corresponding to lanes 1 through 4 respectively.
RPA32 antibody detects RPA32 protein by western blot analysis. Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and blotted with RPA32 antibody (GTX70243) diluted by 1:500. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.