Human CBFb (core-binding factor, beta subunit) using two KLH-conjugated synthetic peptides containing sequences from the central region of the protein.
亚型:
IgG
形态:
Liquid
保存条件:
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
克隆性:
Polyclonal
标记物:
Unconjugated
适应物种:
Human
保质期:
12 months from the shipping date of the product.
抗原来源:
Human
目录编号:
GTX60333
级别:
Primary Antibodies
库存:
Available
供应商:
GeneTex
宿主:
Rabbit
应用范围:
ELISA, ChIP assay
靶点:
CBFb
抗体英文名:
CBFb antibody - ChIP grade
抗体名:
CBFb 抗体 - ChIP grade
规格:
50 μl
ELISA analysis of peptides used for immunization using GTX60333 CBFb antibody - ChIP grade.
ChIP analysis of SKNO-1 cells using GTX60333 CBFb antibody - ChIP grade. The IP'd DNA from 6 ChIP's was pooled and analysed with an Illumina Genome Analyzer. Library preparation, cluster generation and sequencing were performed according to the manufacturer's instructions. The 32 bp tags were aligned to the human reference genome (hg18) using the ELAND algorithm. Figure 2 shows the results of the complete chromosome 3 and three genomic regions region surrounding the OGG1, FUT7 and NFE2 genes, respectively. The position of the PCR amplicon is indicated with an arrow.
ChIP analysis of sheared chromatin from 1.25x10⁶ SKNO-1 cells using GTX60333 CBFb antibody - ChIP grade. QPCR was performed using primers specific for the FUT7, OGG1, NFE2, and SPI1 genes. This figure shows the relative occupancy, calculated as the ratio + control/background for which the MYOG gene was used. Antibody amount : 4μl