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- 详细信息
- 文献和实验
- 技术资料
- 免疫原:
Recombinant protein encompassing a sequence within the center region of human RPA32. The exact sequence is proprietary.
- 亚型:
IgG
- 形态:
Liquid
- 保存条件:
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
- 克隆性:
Polyclonal
- 标记物:
Unconjugated
- 适应物种:
Human, Mouse, Rat
- 保质期:
12 months from the shipping date of the product.
- 抗原来源:
Human
- 目录编号:
GTX113004
- 级别:
Primary Antibodies
- 库存:
Available
- 供应商:
GeneTex
- 宿主:
Rabbit
- 应用范围:
WB, ICC/IF, IHC-P
- 浓度:
1.64 mg/ml (Please refer to the vial label for the specific concentration.)
- 靶点:
RPA32
- 抗体英文名:
RPA32 antibody
- 抗体名:
RPA32 抗体
- 规格:
100 μl/25 μl
| 规格: | 100 μl | 产品价格: | ¥4000.0 |
|---|---|---|---|
| 规格: | 25 μl | 产品价格: | ¥1700.0 |
Whole cell extract (30 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with RPA32 antibody (GTX113004) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
RPA32 antibody detects RPA32 protein at nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% PFA at RT for 15 min.
Green: RPA32 stained by RPA32 antibody (GTX113004) diluted at 1:500.
Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000.
Immunohistochemical analysis of paraffin-embedded A549 Xenograft, using RPA 32 kDa subunit (GTX113004) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
RPA32 antibody detects RPA2 protein by Western blot analysis.
A. 30 μg BCL-1 whole cell lysate/extract
12 % SDS-PAGE
RPA32 antibody (GTX113004) dilution: 1:1000
RPA32 antibody detects RPA2 protein by Western blot analysis.
A. 30 μg PC-12 whole cell lysate/extract
B. 30 μg Rat2 whole cell lysate/extract
12 % SDS-PAGE
RPA32 antibody (GTX113004) dilution: 1:1000
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文献和实验Parisotto M et al., J Exp Med 2018 (PMID:29743291)
The ribonuclease protection assay (RPA)
, 5X transcription buffer, and RPA template set to RT. For each probe synthesis, add the following in order to a 1.5 ml Eppendorf tube: 1 µl RNasin® 1 µl GACU pool 2 µl DTT 4 µl 5X transcription buffer 1 µl RPA Template Set 10 µl [a-32
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人白细胞介素32γ(IL-32γ)酶联免疫分析(ELISA)
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