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- 详细信息
- 用户评价
- 文献和实验
- 技术资料
- 免疫原:
Recombinant protein encompassing a sequence within the center region of human GAPDH. The exact sequence is proprietary.
- 亚型:
IgG2b
- 形态:
Liquid
- 保存条件:
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
- 克隆性:
Monoclonal
- 标记物:
Unconjugated
- 适应物种:
Human, Mouse, Rat, Zebrafish, Yeast, Drosophila, Dog, Pig, Monkey, Candida albicans, E. coli
- 保质期:
12 months from the shipping date of the product.
- 抗原来源:
Human
- 目录编号:
GTX627408
- 级别:
Primary Antibodies
- 库存:
Available
- 供应商:
GeneTex
- 宿主:
Mouse
- 应用范围:
WB, ICC/IF, IHC-P, EMSA
- 浓度:
1 mg/ml (Please refer to the vial label for the specific concentration.)
- 靶点:
GAPDH
- 抗体英文名:
GAPDH antibody [GT239]
- 抗体名:
GAPDH 抗体 [GT239]
- 规格:
100 μl/25 μl
| 规格: | 100 μl | 产品价格: | ¥4000.0 |
|---|---|---|---|
| 规格: | 25 μl | 产品价格: | ¥1700.0 |
Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with GAPDH antibody [GT239] (GTX627408) diluted at 1:10000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.
GAPDH antibody [GT239] detects GAPDH protein by western blot analysis.
A. 20 μg 293T whole cell lysate/extract
B. 10 μg 293T whole cell lysate/extract
C. 5 μg 293T whole cell lysate/extract
D. 1 μg 293T whole cell lysate/extract
10% SDS-PAGE
GAPDH antibody [GT239] (GTX627408) dilution: 1:10000
The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.
GAPDH antibody [GT239] detects GAPDH protein by western blot analysis.
A. 30 μg yeast whole cell lysate/extract
10% SDS-PAGE
GAPDH antibody [GT239] (GTX627408) dilution: 1:10000
The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.
Whole cell extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with GAPDH antibody [GT239] (GTX627408) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.
Sample (30 μg of whole cell lysate)
A: Drosophila
10% SDS PAGE
GTX627408 diluted at 1:5000
The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.
GAPDH antibody [GT239] detects GAPDH protein at cytoplasm by immunofluorescent analysis.
Sample: HeLa cells were fixed in ice-cold MeOH for 5 min.
Green: GAPDH stained by GAPDH antibody [GT239] (GTX627408) diluted at 1:500.
Blue: Hoechst 33342 staining.
Various tissue extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with GAPDH antibody [GT239] (GTX627408) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.
GAPDH antibody [GT239] detects GAPDH protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse kidney.
GAPDH stained by GAPDH antibody [GT239] (GTX627408) diluted at 1:200.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with GAPDH antibody [GT239] (GTX627408) diluted at 1:10000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.
GAPDH antibody [GT239] detects GAPDH protein by western blot analysis.
A. 30 μg 293T whole cell lysate/extract
B. 30 μg NIH-3T3 whole cell lysate/extract
C. 30 μg mouse brain lysate/extract
D. 30 μg PC-12 whole cell lysate/extract
E. 30 μg rat brain lysate/extract
10% SDS-PAGE
GAPDH antibody [GT239] (GTX627408) dilution: 1:10000
The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.
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文献和实验Application Reference
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