- ￥6100
- GeneTex
- 美国
- GTX60358
- 2025年07月13日
- WB, ELISA, ChIP assay
- Rabbit
- Human
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- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
MeCP2 (Methyl-CpG-binding domain protein 2), using a KLH-conjugated synthetic peptide containing a sequence from the C-terminal part of the protein.
- 亚型：
IgG
- 形态：
Liquid
- 保存条件：
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
- 克隆性：
Polyclonal
- 标记物：
Unconjugated
- 适应物种：
Human
- 保质期：
12 months from the shipping date of the product.
- 抗原来源：
Human
- 目录编号：
GTX60358
- 级别：
Primary Antibodies
- 库存：
Available
- 供应商：
GeneTex
- 宿主：
Rabbit
- 应用范围：
WB, ELISA, ChIP assay
- 浓度：
1.2 mg/ml (Please refer to the vial label for the specific concentration.)
- 靶点：
MECP2
- 抗体英文名：
MECP2 antibody - ChIP grade
- 抗体名：
MECP2 抗体 - ChIP grade
- 规格：
50 μg
ChIP analysis of sheared chromatin from 10⁶ U2OS cells using GTX60358 MECP2 antibody - ChIP grade. IgG (1 μg/IP) was used as a negative IP control. Quantitative PCR was performed with primers for the promoters of the ZMYND10 gene (used as a positive control) and CDC6 gene (used as a negative control). This figure shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).
WB analysis of whole cell extracts (40 μg) from HeLa cells transfected with MeCP2 siRNA (lane 2) and from an untransfected control (lane 1) using GTX60358 MECP2 antibody - ChIP grade.
Dilution : 1:1,000
ELISA analysis of peptides used for immunization using GTX60358 MECP2 antibody - ChIP grade.
WB analysis of nuclear extracts (25 μg) from HeLa cells using GTX60358 MECP2 antibody - ChIP grade.
Dilution : 1:1,000
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文献和实验Lee LC et al., Genes Brain Behav 2019 (PMID:30806012)
Herring Sperm DNA37% Formaldehyde (ACS reagent grade)1.25 M glycineProtocol: (Generalized for all cell types- inquire for details regarding particular cell types) For all the following steps,use the pipets that are specifically designated for ChIP use
Characterization and Quality Control of Antibodies Used in ChIP Assays
, modifying enzymes, and chromatin-interacting proteins. The final purpose of the characterization and QC is to label antibodies as chromatin immunoprecipitation (ChIP) grade. Indeed, the ChIP method is extensively used in epigenetics to study gene regulation
ChIP‐chip for Genome‐Wide Analysis of Protein Binding in Mammalian Cells
Abstract Table of Contents Materials Figures Literature Cited Abstract ChIP?chip combines
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