- ￥1700 - 4000
- GeneTex
- 美国
- GTX128957
- 2025年07月15日
- WB, ICC/IF, IHC-P, IP, Dot, ChIP assay, IHC (Free Floating)
- Rabbit
- Human, Mouse, Rat
企业认证
相关产品推荐更多 >
![beta Tubulin antibody [TU-06]](https://img1.dxycdn.com/2022/0328/452/2034512552120400453-14.jpg!wh200)
![SOS1 antibody [SOS-01]](https://img1.dxycdn.com/2022/0328/615/2380555621685500453-14.jpg!wh200)
万千商家帮你免费找货
0 人在求购买到急需产品
- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
Carrier-protein conjugated synthetic peptide surrounding acetyl Lys8 of human Histone H4. The exact sequence is proprietary.
- 亚型：
IgG
- 形态：
Liquid
- 保存条件：
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
- 克隆性：
Polyclonal
- 标记物：
Unconjugated
- 适应物种：
Human, Mouse, Rat
- 保质期：
12 months from the shipping date of the product.
- 抗原来源：
Human
- 目录编号：
GTX128957
- 级别：
Primary Antibodies
- 库存：
Available
- 供应商：
GeneTex
- 宿主：
Rabbit
- 应用范围：
WB, ICC/IF, IHC-P, IP, Dot, ChIP assay, IHC (Free Floating)
- 浓度：
1 mg/ml (Please refer to the vial label for the specific concentration.)
- 靶点：
Histone H4K8ac (acetyl Lys8)
- 抗体英文名：
Histone H4K8ac (acetyl Lys8) antibody
- 抗体名：
Histone H4K8ac (acetyl Lys8) 抗体
- 规格：
100 μl/25 μl
| 规格： | 100 μl | 产品价格： | ￥4000.0 |
|---|---|---|---|
| 规格： | 25 μl | 产品价格： | ￥1700.0 |
Histone H4 (acetyl Lys8) antibody detects Histone H4 (acetyl Lys8) protein at nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% PFA at RT for 15 min.
Green: Histone H4 (acetyl Lys8) protein stained by Histone H4 (acetyl Lys8) antibody (GTX128957) diluted at 1:500.
Red: alpha Tubulin antibody [GT114], a cytoskeleton marker, stained by GTX628802 diluted at 1:500.
Blue: Hoechst 33342 staining.
Histone H4 (acetyl Lys8) antibody detects Histone H4 (acetyl Lys8) protein by Western blot analysis.
A. 30 μg HeLa whole cell lysate/extract (0.012% DMSO treatment for 18 hr)
B. 30 μg HeLa whole cell lysate/extract (0.4 μM Trichostatin A treatment for 18 hr)
15 % SDS-PAGE
Histone H4 (acetyl Lys8) antibody (GTX128957) dilution: 1:5000
Histone H4K8ac (acetyl Lys8) antibody detects Histone H4K8ac (acetyl Lys8) protein at nucleus on rat hind brain by immunohistochemical analysis.
Sample: Paraffin-embedded rat hind brain.
Histone H4K8ac (acetyl Lys8) antibody (GTX128957) dilution: 1:500.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
Cross-linked ChIP was performed with HeLa chromatin extract treated with Trichostatin A (0.4 μM for 18 h) and 5 μg of either control rabbit IgG or anti-Histone H4K8ac (acetyl Lys8) antibody. The precipitated DNA was detected by PCR with primer set targeting to GAPDH or TEXT12.
Histone H4 (acetyl K9) antibody immunoprecipitates Histone H4 (acetyl K9) protein in IP experiments. IP Sample: HeLa whole cell lysate/extract A : 30 μg whole cell lysate/extract of Histone H4 (acetyl K9) protein expressing HeLa cells (0.4uM TSA treatment for 18hr) B : Control with 2.5 μg of pre-immune rabbit IgG C : Immunoprecipitation of Histone H4 (acetyl K9) protein by 2.5 μg of Histone H4 (acetyl K9) antibody (GTX128957). 12% SDS-PAGE The immunoprecipitated Histone H4 (acetyl K9) protein was detected by Histone H4 (acetyl K9) antibody (GTX128957) diluted at 1 : 5000. EasyBlot anti-rabbit IgG (HRP) (GTX221666-01) was used as a secondary reagent.
Histone H4K8ac (acetyl Lys8) antibody detects Histone H4K8ac (acetyl Lys8) protein at nucleus on mouse kidney by immunohistochemical analysis.
Sample: Paraffin-embedded mouse kidney.
Histone H4K8ac (acetyl Lys8) antibody (GTX128957) dilution: 1:500.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
Dotblot analysis of anti-Histone H4 (acetyl Lys9) antibody with peptide samples.
Varied amount of peptide samples were spotted onto positively charged nylon membrane and blotted with Histone H4 (acetyl Lys9) antibody (GTX128957) 1:5000 dilution.
A: Peptide samples of H4K8 un
B: Peptide samples of H4K8ac
C: Peptide samples of H4K16un
D: Peptide samples of H4K16ac
Histone H4K8ac (acetyl Lys8) antibody detects Histone H4K8ac (acetyl Lys8) protein at nucleus on mouse kidney by immunohistochemical analysis.
Sample: Paraffin-embedded mouse kidney.
Histone H4K8ac (acetyl Lys8) antibody (GTX128957) dilution: 1:500.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
风险提示：丁香通仅作为第三方平台，为商家信息发布提供平台空间。用户咨询产品时请注意保护个人信息及财产安全，合理判断，谨慎选购商品，商家和用户对交易行为负责。对于医疗器械类产品，请先查证核实企业经营资质和医疗器械产品注册证情况。
文献和实验Histone Deacetylase Activity Assay
Histone deacetylases (HDACs) are enzymes that catalyze the removal of acetyl groups from the ɛ-amino groups of conserved lysine residues in the amino terminal tail of histones. In humans, there are 18 potential deacetylase enzymes
组蛋白是和染色体相联的最主要的蛋白质。它的作用是和染色体中的 DNA 的负电荷结合。组蛋白是比较小的碱性蛋白；在细胞正常 pH 值时，组蛋白带有正电荷，这样它们就可以和 DNA 结合，这个正电荷主要存在于碱性氨基酸 Lys 和 Arg 的 -NH3 上。其实，组蛋白约含 25% 的 Arg 和 Lys 。比其他蛋白的 Arg ， Lys 的含量都多。和真核 DNA 结合的有 5 种类型的组蛋白： H1 ， H2A ， H2B ， H3
PROTOCOL To 1.5 mL eppendorf tubes add: 200 µg of protein extract (see Western blot protocol for protein sample preps) q.s. to 300 µL with RIPA (with protease and phosphatase inhibitors). Primary antibody (amount determined
技术资料暂无技术资料 索取技术资料
