Recombinant protein encompassing a sequence within the center region of human SMYD3. The exact sequence is proprietary.
亚型:
IgG
形态:
Liquid
保存条件:
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
克隆性:
Polyclonal
标记物:
Unconjugated
适应物种:
Human, Mouse
保质期:
12 months from the shipping date of the product.
抗原来源:
Human
目录编号:
GTX121945
级别:
Primary Antibodies
库存:
Available
供应商:
GeneTex
宿主:
Rabbit
应用范围:
WB, ICC/IF, IP, ChIP assay
浓度:
1.23 mg/ml (Please refer to the vial label for the specific concentration.)
靶点:
SMYD3
抗体英文名:
SMYD3 antibody
抗体名:
SMYD3 抗体
规格:
100 μl/25 μl
规格:
100 μl
产品价格:
¥4000.0
规格:
25 μl
产品价格:
¥1700.0
Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with SMYD3 antibody (GTX121945) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Immunoprecipitation of SMYD3 protein from 293T whole cell extracts using 5 μg of SMYD3 antibody (GTX121945). Western blot analysis was performed using SMYD3 antibody (GTX121945) diluted at 1:500. EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.
Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with SMYD3 antibody (GTX121945) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Cross-linked ChIP was performed with HepG2 chromatin extract and 5 μg of either normal rabbit IgG or anti-SMYD3 antibody. The precipitated DNA was detected by PCR with primer set targeting to 5' flanking region of Nkx2.8..
SMYD3 antibody detects SMYD3 protein at cytoplasm by immunofluorescent analysis. Sample: HCT116 cells were fixed in ice-cold MeOH for 5 min. Green: SMYD3 protein stained by SMYD3 antibody (GTX121945) diluted at 1:500. Blue: Hoechst 33342 staining.
SMYD3 antibody detects SMYD3 protein by Western blot analysis. Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with SMYD3 antibody (GTX121945) diluted at a dilution of 1:1000.
Application Reference
Proserpio V et al., Genes Dev 2013 (PMID:23752591)
Fittipaldi R et al., Cells 2021 (PMID:34069776)
Yang D et al., Aging (Albany NY) 2020 (PMID:33144524)
Fenizia C et al., Nucleic Acids Res 2019 (PMID:30544196)
Chen YJ et al., Sci Rep 2017 (PMID:28630472)