- ￥5300
- GeneTex
- 美国
- GTX48805
- 2025年10月05日
- WB, ELISA
- Rabbit
- Borrelia burgdorferi
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- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
MBP-fusion protein corresponding to Borrelia burgdorferi p35 protein.
- 亚型：
IgG
- 形态：
Liquid
- 保存条件：
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
- 克隆性：
Polyclonal
- 标记物：
Unconjugated
- 适应物种：
Borrelia burgdorferi
- 保质期：
12 months from the shipping date of the product.
- 抗原来源：
Borrelia burgdorferi
- 目录编号：
GTX48805
- 级别：
Primary Antibodies
- 库存：
Available
- 供应商：
GeneTex
- 宿主：
Rabbit
- 应用范围：
WB, ELISA
- 浓度：
1 mg/ml (Please refer to the vial label for the specific concentration.)
- 靶点：
Borrelia burgdorferi p35
- 抗体英文名：
Borrelia burgdorferi p35 antibody
- 抗体名：
Borrelia burgdorferi p35 抗体
- 规格：
50 μg
Western Blot of Rabbit anti-p35 antibody. Lane 1: p35 recombinant protein 50 ng load. Lane 2: p35 recombinant protein 100 ng load. Lane 3: p35 recombinant protein 250 ng load. Lane 4: p35 recombinant protein 500 ng load. Primary antibody: p35 antibody at 1:1,000 for overnight at 4ºC. Secondary antibody: Peroxidase rabbit secondary antibody at 1:40,000 for 30 min at RT. Blocking for 30 min at RT. Predicted/Observed size: 60 kDa, 60 kDa for p35.
Western blot showing detection of 0.1 μg of recombinant p35 protein. Lane 1: Molecular weight markers. Lane 2: MBP-p35 fusion protein (arrow; expected MW: 69.5 kDa). Lane 3: MBP alone. Protein was run on a 4-20% gel, then transferred to 0.45 μm nitrocellulose. After blocking with 1% BSA-TTBS overnight at 4ºC, primary antibody was used at 1:1000 at room temperature for 30 min. HRP-conjugated Goat-Anti-Rabbit secondary antibody was used at 1:40,000 in blocking buffer and imaged on the VersaDoc MP 4000 imaging system (Bio-Rad).
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文献和实验Electrotransformation of the Spirochete Borrelia burgdorferi
Borrelia burgdorferi is an etiologic agent of Lyme disease, the most common arthropod-borne disease in the United States (1 ,2 ). The bacterium, a member of the spirochete phylum, has a genome predominantly composed of linear DNA molecules
Determining the Cellular Targets of Reactive Oxygen Species in Borrelia burgdorferi
The response of Borrelia burgdorferi to the challenge of reactive oxygen species (ROS) is a direct result of its limited biosynthetic capabilities and lack of biologically significant levels of intracellular Fe. In other bacteria
科学家提出，让通过虱子传播的莱姆病病源体——莱姆病螺旋体（Borrelia burgdorferi）的一个基因不再活动可以阻止这种疾病的传播。此前的研究表明莱姆病螺旋体的bba64基因在进食的虱子体内表达，然而这种基因的功能尚不为人所知。 Robert Gilmore, Jr及其同事为小鼠注射了一种莱姆病螺旋体菌株，这种修改后的菌株的bba64基因被关闭了，然后让虱子以小鼠为食并获得了这种细菌。 这组科学家然后把这些虱子转移给了没有莱姆病的虱子，结果发现8周后15只实验小鼠只有2只感染
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