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6X His tag antibody [RM146]

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  • ¥5800
  • GeneTex已认证
  • 美国
  • GTX33607
  • 2025年07月11日
  • WB, ICC/IF, FACS, IP, IHC
  • Rabbit
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    • 详细信息
    • 文献和实验
    • 技术资料
    • 免疫原

      Mixture of a peptide with 6xHis-Tag at the N-terminus and another peptide with 6xHis-Tag at the C-terminus.

    • 亚型

      IgG

    • 形态

      Liquid

    • 保存条件

      Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

    • 克隆性

      Monoclonal

    • 标记物

      Unconjugated

    • 保质期

      12 months from the shipping date of the product. 

    • 目录编号

      GTX33607

    • 级别

      Primary Antibodies

    • 库存

      Available

    • 供应商

      GeneTex

    • 宿主

      Rabbit

    • 应用范围

      WB, ICC/IF, FACS, IP, IHC

    • 浓度

      Batch dependent (Please refer to the vial label for the specific concentration.)

    • 靶点

      This antibody reacts to recombinant proteins containing the 6xHis-Tag or 10xHis-Tag fused to either the amino or carboxy terminus. No cross reactivity with other endogenous protein in mammalian or bacteria cells.

    • 抗体英文名

      6X His tag antibody [RM146]

    • 抗体名

      6X His tag 抗体 [RM146]

    • 规格

      100 μg

    产品细节图片1

    ICC/IF analysis of 293T cells expressing His-Tag nuclear protein X using GTX33607 6X His tag antibody [RM146].

    产品细节图片2

    WB analysis of various sample lysayes using GTX33607 6X His tag antibody [RM146].
    Lane 1 : 293T cells
    Lane 2 : 293T cells transfected with His-Tag fusion protein X
    Lane 3 : E. coli lysate without His-Tag Protein Y
    Lane 4 : E. coli lysate with His-Tag Protein Y
    Dilution : 0.2μg/ml

    产品细节图片3

    IP analysis of 293T cells expressing a His-Tag fusion protein using GTX33607 6X His tag antibody [RM146].
    Lane 1 : Whole lysate control
    Lane 2 : IP by rabbit IgG control
    Lane 3 : IP by Primary antibody

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    图标文献和实验
    相关实验
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      is added once again and washed extensively with 6X SSPE and 0.01% Tween-20 at 30 °C. The SAPE and antibody were added to arrays in 6X SSPE, 1X Denhardt’s solution and 0.01% Tween-20 at 25 °C for 10 minutes each. Following the final wash, the arrays are kept

    • Geno typing using Affymetrix arrays

      C. The SAPE and antibody were added to arrays in 6X SSPE, 1X Denhardt’s solution and 0.01% Tween-20 at 25 °C for 10 minutes each. Following the final wash, the arrays are kept in Holding buffer (100mM MES, 1M [Na ], 0.01% Tween-20). The washing

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