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- 详细信息
- 文献和实验
- 技术资料
- 免疫原:
Mixture of a peptide with 6xHis-Tag at the N-terminus and another peptide with 6xHis-Tag at the C-terminus.
- 亚型:
IgG
- 形态:
Liquid
- 保存条件:
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
- 克隆性:
Monoclonal
- 标记物:
Unconjugated
- 保质期:
12 months from the shipping date of the product.
- 目录编号:
GTX33607
- 级别:
Primary Antibodies
- 库存:
Available
- 供应商:
GeneTex
- 宿主:
Rabbit
- 应用范围:
WB, ICC/IF, FACS, IP, IHC
- 浓度:
Batch dependent (Please refer to the vial label for the specific concentration.)
- 靶点:
This antibody reacts to recombinant proteins containing the 6xHis-Tag or 10xHis-Tag fused to either the amino or carboxy terminus. No cross reactivity with other endogenous protein in mammalian or bacteria cells.
- 抗体英文名:
6X His tag antibody [RM146]
- 抗体名:
6X His tag 抗体 [RM146]
- 规格:
100 μg
ICC/IF analysis of 293T cells expressing His-Tag nuclear protein X using GTX33607 6X His tag antibody [RM146].
WB analysis of various sample lysayes using GTX33607 6X His tag antibody [RM146].
Lane 1 : 293T cells
Lane 2 : 293T cells transfected with His-Tag fusion protein X
Lane 3 : E. coli lysate without His-Tag Protein Y
Lane 4 : E. coli lysate with His-Tag Protein Y
Dilution : 0.2μg/ml
IP analysis of 293T cells expressing a His-Tag fusion protein using GTX33607 6X His tag antibody [RM146].
Lane 1 : Whole lysate control
Lane 2 : IP by rabbit IgG control
Lane 3 : IP by Primary antibody
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文献和实验Genotyping using Affymetrix arrays
is added once again and washed extensively with 6X SSPE and 0.01% Tween-20 at 30 °C. The SAPE and antibody were added to arrays in 6X SSPE, 1X Denhardt’s solution and 0.01% Tween-20 at 25 °C for 10 minutes each. Following the final wash, the arrays are kept
Geno typing using Affymetrix arrays
C. The SAPE and antibody were added to arrays in 6X SSPE, 1X Denhardt’s solution and 0.01% Tween-20 at 25 °C for 10 minutes each. Following the final wash, the arrays are kept in Holding buffer (100mM MES, 1M [Na ], 0.01% Tween-20). The washing
在于遗传背景稳定,操作方便且经济。RA 模型大致可分为诱导型和转基因型两大类,本文将针对这两大类模型进行总结和比较,以便广大学者为自己的研究选择合适的 RA 模型。 一、诱导型类风湿关节炎模型 目前,最常见的诱导型类风湿关节炎模型包括佐剂诱导关节炎(Adjuvant arthritis,AA)、胶原诱导的关节炎(Collagen-induced Arthritis, CIA)、胶原抗体诱导的关节炎(Collagen antibody-induced Arthritis, CAIA)以及其他的药物
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