- ￥1700 - 4000
- GeneTex
- 美国
- GTX124250
- 2025年07月14日
- WB, ICC/IF, FACS
- Rabbit
- Dengue virus
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- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
Full length Dengue virus 2 NS4B Recombinant protein (Dengue virus 2 (strain 16681 PDK 53))
- 亚型：
IgG
- 形态：
Liquid
- 保存条件：
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
- 克隆性：
Polyclonal
- 标记物：
Unconjugated
- 适应物种：
Dengue virus
- 保质期：
12 months from the shipping date of the product.
- 抗原来源：
Dengue virus 2
- 目录编号：
GTX124250
- 级别：
Primary Antibodies
- 库存：
Available
- 供应商：
GeneTex
- 宿主：
Rabbit
- 应用范围：
WB, ICC/IF, FACS
- 浓度：
1 mg/ml (Please refer to the vial label for the specific concentration.)
- 靶点：
Dengue virus NS4B protein
- 抗体英文名：
Dengue virus NS4B protein antibody
- 抗体名：
Dengue virus NS4B protein 抗体
- 规格：
100 μl/25 μl
| 规格： | 100 μl | 产品价格： | ￥4000.0 |
|---|---|---|---|
| 规格： | 25 μl | 产品价格： | ￥1700.0 |
NS4B (Dengue virus) antibody detects NS4B (Dengue virus) protein at cytoplasm by immunofluorescent analysis.
Samples: BHK-21 cells mock (left) and infected with Dengue virus 2 (right) were fixed in MeOH.
Green: NS4B (Dengue virus) protein stained by NS4B (Dengue virus) antibody (GTX124250) diluted at 1:2000.
Blue: Hoechst 33342 staining.
Non-infected (–) and infected (+) C6/36 whole cell extracts (15 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with Dengue virus NS4B protein antibody (GTX124250) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Immunofluorescence analysis of PFA-fixed BHK-21 cell, using NS4B (Dengue virus 2)(GTX124250) antibody at 1:2000 dilution.
NS4B (Dengue virus) antibody detects NS4B (Dengue virus) protein at cytoplasm by immunofluorescent analysis.
Samples: BHK-21 cells mock (left) and infected with Dengue virus 3 (right) were fixed in MeOH.
Green: NS4B (Dengue virus) protein stained by NS4B (Dengue virus) antibody (GTX124250) diluted at 1:2000.
Blue: Hoechst 33342 staining.
Sample (20 μg of whole cell lysate)
A: BHK-21
B: Dengue virus 2 infect BHK-21
C: JEV infect BHK-21
10% SDS PAGE
GTX124250 diluted at 1:2000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
NS4B (Dengue virus) antibody detects NS4B (Dengue virus) protein by western blot analysis.
A. 30 μg BHK-21 whole cell extract
B. 30 μg whole cell extract of Dengue virus type 2 infected BHK-21 cells
C. 30 μg whole cell extract of Dengue virus type 3 infected BHK-21 cells
12% SDS-PAGE
NS4B (Dengue virus) antibody (GTX124250) dilution: 1:2000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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文献和实验Tsai TT et al., PLoS Negl Trop Dis 2014 (PMID:25412261)
Wu YW et al., Sci Rep 2016 (PMID:27558165)
Ho MR et al., Sci Rep 2017 (PMID:28761128)
Poh MK et al., Antiviral Res 2012 (PMID:22146564)
Shwetha Shivaprasad et al., PLoS Pathog 2022 (PMID:35482886)
Sarah Goellner et al., STAR Protoc 2020 (PMID:33377067)
Wesley Freppel et al., Bio Protoc 2018 (PMID:34532557)
Mazeaud C et al., Viruses 2021 (PMID:34696522)
Loe MWC et al., Antiviral Res 2020 (PMID:33080251)
Cerikan B et al., Cell Rep 2020 (PMID:32640225)
Mohamed B et al., Sci Rep 2020 (PMID:32132633)
Ngo AM et al., Elife 2019 (PMID:31516121)
Lee JK et al., Antiviral Res 2019 (PMID:31421165)
P?aszczyca A et al., PLoS Pathog 2019 (PMID:31071189)
Scaturro P et al., Nature 2018 (PMID:30177828)
Roth H et al., MBio 2017 (PMID:28074025)
Reid DW et al., J Virol 2018 (PMID:29321322)
Hsieh MS et al., PLoS One 2017 (PMID:29161301)
Sepulveda-Salinas KJ et al., Cell Stress Chaperones 2017 (PMID:28755304)
Tsai TT et al., Antiviral Res 2017 (PMID:28733114)
Fang YT et al., PLoS One 2014 (PMID:25329914)
against dengue, timely diagnosis is therefore necessary for patient management. Laboratory diagnosis is carried out by virus isolation, demonstration of viral antigen, presence of viral nucleic acid, and antibodies. Further, recombinant dengue envelope protein
Characterization of the Dengue Virus Envelope Glycoprotein Expressed in Pichia pastoris
The full-length and truncated forms of recombinant envelope (E) glycoprotein from Dengue virus type 1, Singapore strain S275/90 were expressed in the yeast, Pichia pastoris , using a secretory vector. A truncated form of the E protein
The nonstructural protein 5 (NS5) of dengue virus (DENV) plays a central role in the virus replication. It functions as a methyltransferase and an RNA-dependent RNA polymerase. As such, it is a promising target for antiviral drug development
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