- ￥1700 - 4000
- GeneTex
- 美国
- GTX103349
- 2025年07月12日
- WB, ICC/IF, FACS
- Rabbit
- Dengue virus
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- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
Carrier-protein conjugated synthetic peptide encompassing a sequence within the N-terminus region of Dengue virus NS4B protein (Dengue virus 2 (strain 16681 PDK 53)). The exact sequence is proprietary.
- 亚型：
IgG
- 形态：
Liquid
- 保存条件：
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
- 克隆性：
Polyclonal
- 标记物：
Unconjugated
- 适应物种：
Dengue virus
- 保质期：
12 months from the shipping date of the product.
- 抗原来源：
Dengue virus 2
- 目录编号：
GTX103349
- 级别：
Primary Antibodies
- 库存：
Available
- 供应商：
GeneTex
- 宿主：
Rabbit
- 应用范围：
WB, ICC/IF, FACS
- 浓度：
1 mg/ml (Please refer to the vial label for the specific concentration.)
- 靶点：
Dengue virus NS4B protein
- 抗体英文名：
Dengue virus NS4B protein antibody
- 抗体名：
Dengue virus NS4B protein 抗体
- 规格：
100 μl/25 μl
| 规格： | 100 μl | 产品价格： | ￥4000.0 |
|---|---|---|---|
| 规格： | 25 μl | 产品价格： | ￥1700.0 |
Sample(15 μg of cell lysate)
A: BHK21
B: DV infected BHK21
10% SDS PAGE
GTX103349 diluted at 1:5000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
NS4B (Dengue virus) antibody detects NS4B (Dengue virus) protein at cytoplasm by immunofluorescent analysis.
Samples: BHK-21 cells mock (left) and infected with Dengue virus 2 (right) were fixed in MeOH.
Green: NS4B (Dengue virus) protein stained by NS4B (Dengue virus) antibody (GTX103349) diluted at 1:2000.
Blue: Hoechst 33342 staining.
NS4B (Dengue virus) antibody detects NS4B (Dengue virus) protein at cytoplasm by immunofluorescent analysis.
Samples: BHK-21 cells mock (left) and infected with Dengue virus 3 (right) were fixed in MeOH.
Green: NS4B (Dengue virus) protein stained by NS4B (Dengue virus) antibody (GTX103349) diluted at 1:2000.
Blue: Hoechst 33342 staining.
NS4B (Dengue virus) antibody detects NS4B (Dengue virus) protein by western blot analysis.
A. 30 μg BHK-21 whole cell extrac
B. 30 μg whole cell extract of Dengue virus type 2 infected BHK-21 cells
C. 30 μg whole cell extract of Dengue virus type 3 infected BHK-21 cells
12% SDS-PAGE
NS4B (Dengue virus) antibody (GTX103349) dilution: 1:5000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
GTX103349 IF Image Immunofluorescence analysis of Denque virus 2-infected BHK-21, using Non-structural protein 4B (Dengue virus 2 ) antibody (GTX103349) at 1:2000 dilution.
Non-infected (–) and infected (+) C6/36 whole cell extracts (15 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with Dengue virus NS4B protein antibody (GTX103349) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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文献和实验Barbier V et al., Virology 2017 (PMID:27816895)
Tian JN et al., Viruses 2019 (PMID:31461934)
Lin DL et al., Cell Rep 2019 (PMID:31067454)
Zhang J et al., Cell Host Microbe 2018 (PMID:29902443)
Hafirassou ML et al., Cell Rep 2017 (PMID:29281836)
Perry JW et al., J Virol 2018 (PMID:29321321)
Naik NG et al., J Virol 2015 (PMID:25878113)
Udawatte DJ et al., Front Cell Infect Microbiol 2022 (PMID:36310878)
Shen TJ et al., JCI Insight 2022 (PMID:36125898)
against dengue, timely diagnosis is therefore necessary for patient management. Laboratory diagnosis is carried out by virus isolation, demonstration of viral antigen, presence of viral nucleic acid, and antibodies. Further, recombinant dengue envelope protein
Characterization of the Dengue Virus Envelope Glycoprotein Expressed in Pichia pastoris
The full-length and truncated forms of recombinant envelope (E) glycoprotein from Dengue virus type 1, Singapore strain S275/90 were expressed in the yeast, Pichia pastoris , using a secretory vector. A truncated form of the E protein
The nonstructural protein 5 (NS5) of dengue virus (DENV) plays a central role in the virus replication. It functions as a methyltransferase and an RNA-dependent RNA polymerase. As such, it is a promising target for antiviral drug development
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