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- 详细信息
- 文献和实验
- 技术资料
- 免疫原:
Recombinant protein encompassing a sequence within the N-terminus region of human GAD67. The exact sequence is proprietary.
- 亚型:
IgG
- 形态:
Liquid
- 保存条件:
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
- 克隆性:
Polyclonal
- 标记物:
Unconjugated
- 适应物种:
Human, Mouse, Rat, Zebrafish
- 保质期:
12 months from the shipping date of the product.
- 抗原来源:
Human
- 目录编号:
GTX101881
- 级别:
Primary Antibodies
- 库存:
Available
- 供应商:
GeneTex
- 宿主:
Rabbit
- 应用范围:
WB, ICC/IF, IHC-P, IHC-Fr, IHC-Wm, IHC, IHC (Free Floating)
- 浓度:
0.22 mg/ml (Please refer to the vial label for the specific concentration.)
- 靶点:
This antibody is specific for GAD67 protein, and it does not cross-react with GAD65 protein.
- 抗体英文名:
GAD67 antibody
- 抗体名:
GAD67 抗体
- 规格:
100 μl/25 μl
| 规格: | 100 μl | 产品价格: | ¥4000.0 |
|---|---|---|---|
| 规格: | 25 μl | 产品价格: | ¥1700.0 |
Various tissue extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with GAD67 antibody (GTX101881) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
GAD67 antibody detects GAD67 protein on myofiber membranes by immunohistochemical analysis.
Sample: Agarose-embedded zebrafish embryo
GAD67 antibody (GTX101881) dilution: 1:200.
Image provided with permission courtesy of Dr. T. Schilling at UC, Irvine.
GAD67 antibody detects GAD67 protein on myofiber membranes by immunohistochemical analysis.
Sample: Whole-mount zebrafish embryo
GAD67 antibody (GTX101881) dilution: 1:200.
Human tissue extract (30 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with GAD67 antibody (GTX101881) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Various tissue extracts (50 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with GAD67 antibody (GTX101881) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
GAD67 antibody detects GAD67 protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded rat brain.
GAD67 stained by GAD67 antibody (GTX101881) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with GAD67 antibody (GTX101881) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
GAD67 antibody detects GAD67 protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse brain.
GAD67 stained by GAD67 antibody (GTX101881) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
GAD67 antibody detects GAD67 protein by immunofluorescent analysis.
Sample: Mock and transfected 293T cells were fixed in 4% PFA at RT for 15 min.
Green: GAD67 stained by GAD67 antibody (GTX101881) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).
GAD67 antibody detects GAD67 protein by immunofluorescent analysis.
Sample: DIV10 rat E18 primary cortical neuron cells were fixed in 4% PFA at RT for 15 min.
Green: GAD67 stained by GAD67 antibody (GTX101881) diluted at 1:500.
Red: Tau, stained by Tau antibody [GT287] (GTX634809) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).
GAD67 antibody detects GAD67 protein at cell membrane and cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse eye.
Green: GAD67 stained by GAD67 antibody (GTX101881) diluted at 1:250.
Red: beta Tubulin 3/ Tuj1, a neural marker, stained by beta Tubulin 3/ Tuj1 antibody [GT11710] (GTX631836) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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文献和实验Occelli F et al., Neuroscience 2019 (PMID:30769096)
Zhou Z et al., Mol Neurobiol 2014 (PMID:24973145)
Tsai CW et al., Neurotherapeutics 2022 (PMID:35229268)
Ji MH et al., Brain Behav Immun 2020 (PMID:32505714)
Ma X et al., J Endocrinol 2020 (PMID:32698150)
Wang C et al., Neuroscience 2019 (PMID:30703506)
Generation of Antibody Molecules Through Antibody Engineering
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General comments: Antibodies, like most proteins, do not like to be freeze-thawed. Avoid repetitive freezing of your solution. The best way to store your antibody is to keep a high protein concentration (>1 mg/ml), add some protease
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