- ￥1700 - 4000
- GeneTex
- 美国
- GTX110267
- 2025年07月16日
- WB, ICC/IF, IHC-P, IP
- Rabbit
- Human, Mouse, Rat
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- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
Recombinant protein encompassing a sequence within the center region of human Glycine dehydrogenase. The exact sequence is proprietary.
- 亚型：
IgG
- 形态：
Liquid
- 保存条件：
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
- 克隆性：
Polyclonal
- 标记物：
Unconjugated
- 适应物种：
Human, Mouse, Rat
- 保质期：
12 months from the shipping date of the product.
- 抗原来源：
Human
- 目录编号：
GTX110267
- 级别：
Primary Antibodies
- 库存：
Available
- 供应商：
GeneTex
- 宿主：
Rabbit
- 应用范围：
WB, ICC/IF, IHC-P, IP
- 浓度：
0.46 mg/ml (Please refer to the vial label for the specific concentration.)
- 靶点：
Glycine dehydrogenase
- 抗体英文名：
Glycine dehydrogenase antibody [N3C2-2], Internal
- 抗体名：
Glycine dehydrogenase 抗体 [N3C2-2], Internal
- 规格：
100 μl/25 μl
| 规格： | 100 μl | 产品价格： | ￥4000.0 |
|---|---|---|---|
| 规格： | 25 μl | 产品价格： | ￥1700.0 |
Whole cell extract (30 μg) was separated by 5% SDS-PAGE, and the membranes were blotted with Glycine dehydrogenase antibody [N3C2-2], Internal (GTX110267) diluted at 1:1000 and competitor's antibody (CST#12794) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Glycine dehydrogenase antibody [N3C2-2], Internal detects Glycine dehydrogenase protein at mitochondria in mouse liver by immunohistochemical analysis.
Sample: Paraffin-embedded mouse liver.
Glycine dehydrogenase antibody [N3C2-2], Internal (GTX110267) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
Glycine dehydrogenase antibody [N3C2-2], Internal detects Glycine dehydrogenase protein at cytoplasm in rat brain by immunohistochemical analysis.
Sample: Paraffin-embedded rat brain.
Glycine dehydrogenase antibody [N3C2-2], Internal (GTX110267) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
Glycine dehydrogenase antibody [N3C2-2], Internal detects Glycine dehydrogenase protein at mitochondria by immunofluorescent analysis.
Sample: Hep G2 cells were fixed in ice-cold MeOH for 5 min.
Green: Glycine dehydrogenase protein stained by Glycine dehydrogenase antibody [N3C2-2], Internal (GTX110267) diluted at 1:400.
Blue: Hoechst 33342 staining.
Immunoprecipitation of Glycine dehydrogenase protein from HepG2 whole cell extracts using 5 μg of Glycine dehydrogenase antibody [N3C2-2], Internal (GTX110267).
Western blot analysis was performed using Glycine dehydrogenase antibody [N3C2-2], Internal (GTX110267).
EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.
Glycine dehydrogenase antibody [N3C2-2], Internal detects Glycine dehydrogenase protein at cytoplasm in mouse brain by immunohistochemical analysis.
Sample: Paraffin-embedded mouse brain.
Glycine dehydrogenase antibody [N3C2-2], Internal (GTX110267) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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文献和实验Protocol for anti-HA antibody Western Blotting
of interest. 2) Transfer proteins in acrylamide gel to nylon membrane. 3) Block overnight @4℃ in PBS (or TBS, it doesn't matter) with 5% non-fat dry milk. I dilute 2.5 mg in 50 ml. 4) Wash off excess milk sol'n with PBS x3 .(important if you are re-using your antibody
Using Biacore to Measure the Binding Kinetics of an Antibody‐Antigen Interaction
?protein interaction is described using an antibody and its antigen as an example. The affinity of the antibody for its antigen is determined by measuring the binding kinetics of the interaction. The protocols are divided into three major steps
secondary antibody review -- data from 99 publications
cytometry used as a control to detect cell responses targeted antigen 7 Alexa Fluor 488 7 Cy3 8 goat IgG Alexa Fluor 488 1:2000 detect antibody binding in human embryonic kidney 293T cells Invitrogen 9 donkey
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