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- 详细信息
- 文献和实验
- 技术资料
- 免疫原:
Carrier-protein conjugated synthetic peptide encompassing a sequence within the N-terminus region of human Bcl-2. The exact sequence is proprietary.
- 亚型:
IgG
- 形态:
Liquid
- 保存条件:
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
- 克隆性:
Polyclonal
- 标记物:
Unconjugated
- 适应物种:
Human, Mouse, Rat, Zebrafish, Cat, Dog
- 保质期:
12 months from the shipping date of the product.
- 抗原来源:
Human
- 目录编号:
GTX100064
- 级别:
Primary Antibodies
- 库存:
Available
- 供应商:
GeneTex
- 宿主:
Rabbit
- 应用范围:
WB, ICC/IF, IHC-P
- 浓度:
0.2 mg/ml (Please refer to the vial label for the specific concentration.)
- 靶点:
Bcl-2
- 抗体英文名:
Bcl-2 antibody [N1N2], N-term
- 抗体名:
Bcl-2 抗体 [N1N2], N-term
- 规格:
100 μl/25 μl
| 规格: | 100 μl | 产品价格: | ¥4000.0 |
|---|---|---|---|
| 规格: | 25 μl | 产品价格: | ¥1700.0 |
Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with Bcl-2 antibody [N1N2], N-term (GTX100064) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with Bcl-2 antibody [N1N2], N-term (GTX100064) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Rat tissue extract (50 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with Bcl-2 antibody [N1N2], N-term (GTX100064) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.
Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with Bcl-2 antibody [N1N2], N-term (GTX100064) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.
Mouse tissue extract (50 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with Bcl-2 antibody [N1N2], N-term (GTX100064) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Bcl-2 antibody [N1N2], N-term detects Bcl-2 protein at cytoplasm in human squamous metaplasia of cervix by immunohistochemical analysis.
Sample: Paraffin-embedded human cervix.
Bcl-2 antibody [N1N2], N-term (GTX100064) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
Immunohistochemical analysis of paraffin-embedded human breast cancer, using Bcl-2(GTX100064) antibody at 1:250 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
Bcl-2 antibody detects Bcl-2 protein at cytoplasm and nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded dog skin.
Bcl-2 stained by Bcl-2 antibody (GTX100064) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
Whole cell extract (30 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with Bcl-2 antibody (GTX100064) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.
PG-4 whole cell extract (30 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with Bcl-2 antibody (GTX100064) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.
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In the field of therapeutic recombinant proteins, monoclonal antibodies (mAbs) have achieved a rising success with more than 30 mAbs that have reached the market in the past 20 years. From a structural standpoint, one of the most important
大鼠凋亡因子BCL-2(BCL-2)ELISA试剂盒 说明书
上海西唐生物科技有限公司 021-55229872, 65333639 www.westang.com 大鼠凋亡因子 BCL-2(BCL-2)ELISA 试剂盒 ( 用于血清、血浆、细胞培养上清液和生物体液内 ) 原理 本实验采用双抗体夹心 ABC-ELISA 法。用抗大鼠 BCL-2 单抗包被于酶标板上,标准品和样品中的 BCL-2 与单抗结合,加入生物素化的抗大鼠 BCL-2 ,形成免疫复合物连接
to protect the antibody. These should be stored in smaller aliquots (about 1 ml) and frozen for long term storage though the protected solution is also stable for months at four degrees. Ascites fluid: This is a bit more problematic as it may contain
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