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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
-20℃ to -80℃
- 保质期:
12个月
- 英文名:
Recombinant Human IL4R / CD124 Protein (His Tag)
- 库存:
99
- 供应商:
北京义翘神州科技股份有限公司
- 规格:
1.00 mg/50.00 µg/100.00 µg/500.00 µg
| 规格: | 1.00 mg | 产品价格: | ¥26795.0 |
|---|---|---|---|
| 规格: | 50.00 µg | 产品价格: | ¥2570.0 |
| 规格: | 100.00 µg | 产品价格: | ¥4520.0 |
| 规格: | 500.00 µg | 产品价格: | ¥15050.0 |
蛋白名称:Human IL4R / CD124 Protein (His Tag)
蛋白构建:A DNA sequence encoding the human IL4R (NP_000409.1) precursor (Met 1-His 232) was fused with a polyhistidine tag at the C-terminus.
表达宿主:HEK293 Cells
蛋白纯度:> 98 % as determined by SDS-PAGE
蛋白活性:1. Measured by its binding ability in a functional ELISA. Immobilized human IL4R-His (Cat:10402-H08H) at 10 μg/mL (100 μl/well) can bind biotinylated human IL4 (Cat:11846-HNAE). The EC50 of biotinylated human IL4 is 20.8-48.5 ng/mL. 2. Measured by its ability to inhibit IL-4 dependent proliferation of TF-1 human erthroleukemic cells. The ED50 for this effect is 2-10 ng/mL in the presence of 2 ng/ml IL-4.
蛋白内毒素:< 1.0 EU per μg of the protein as determined by the LAL method
预测N端:Met 26
蛋白分子量:The secreted recombinant human IL4R consists of 218 amino acids after cleavage of the signal peptide and predictes a molecular mass of 25.18 kDa. In SDS-PAGE under reducing conditions, it migrates with the apparent molecular mass of 43-48 kDa due to glycosylation.
蛋白NP号:NP_000409.1
蛋白氨基酸序列:Met1-His232
蛋白标签:C-His
蛋白保存条件:Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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