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- 文献和实验
- 技术资料
- 保存条件:
-20℃ to -80℃
- 保质期:
12个月
- 英文名:
Human IL1R2 / CD121b Protein (Fc Tag)
- 库存:
99
- 供应商:
北京义翘神州科技股份有限公司
- 规格:
100 µg/1 mg
| 规格: | 100 µg | 产品价格: | ¥3220.0 |
|---|---|---|---|
| 规格: | 1 mg | 产品价格: | ¥21030.0 |
蛋白名称:Human IL1R2 / CD121b Protein (Fc Tag)
蛋白构建:A DNA sequence encoding the extracellular domain (Met 1-Glu 343) of human IL1 receptor type I I (IL1RII) (NP_004624.1) was expressed with C-terminal fused Fc region of human IgG1.
表达宿主:HEK293 Cells
蛋白纯度:> 95 % as determined by SDS-PAGE
蛋白活性:1. Measured by its binding ability in a functional ELISA. Immobilized recombinant human IL1B at 2 μg/ml (100ul/well) can bind recombinant human IL1R2-Fc with a linear range of 6.25-400 ng/ml. 2. Using the Octet RED System, the affinity constant (Kd) of IL1R2 Protein, Human, Recombinant (Fc Tag) (Cat. 10111-H02H) bound IL-1 beta Protein, Human, Recombinant (Cat. 10139-HNAE) was 6.0 nM. 3. Measured by its ability to inhibit Interferon gamma secretion by human natural killer lymphoma NK-92 cells in the presence of 500 pg/mL IL1b. The EC50 for this effect is typically 0.1-0.8 μg/mL.
蛋白内毒素:< 1.0 EU per μg of the protein as determined by the LAL method
预测N端:Phe 14
蛋白分子量:The recombinant human IL1R2/Fc is a disulfide-linked homodimer. The reduced monomer consists of 568 amino acids and has a predicted molecular mass of 64.5 kDa. As a result of glycosylation, the rh IL1R2/Fc monomer migrates as an approximately 70-75 kDa band in SDS-PAGE under reducing conditions.
蛋白NP号:NP_004624.1
蛋白氨基酸序列:Met1-Glu343
蛋白标签:C-human IgG1-Fc
蛋白保存条件:Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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文献和实验1 - - (小鼠) TCRγ/δ限制成分? 免疫球蛋白Fc段受体 polyIgR 100 - 1 4 1q31-q42 多聚Ig受体(肝细胞上 polyIgR是HBV受体) FcγRⅠ(CD64) 70 - 3 - 1q23-q24 IgG Fc高亲和力受体 FcγRⅡ(CD32) 40 - 2 - 1q23-q24 IgG Fc低亲和力受体 FcγRⅢ(CD16) 50~70 - 2 - 1q23-q24 IgG Fc低亲和力受体 FcεRⅠα链 25
x 106/ml; 3.4 加入1 000 U/ml 的重组人IFN-g培养; 3.5 24h 后加入50ng/ml 的CD3 单克隆抗体和300 U/ml 的重组人IL-2,刺激CIK 细胞的生长和增殖; 注:此时也可同时加入100 U/ml的重组人IL-1a。 3.6 每3天半量换液或扩瓶一次,并补加重组人IL-2 300 U/ml; 3.7 在培养的第7d,收获CIK细胞,此时数量应达到1x 109个以上。 3.8 CIK细胞质控: 3.8.1 台盼蓝染色检测
、ionomycin和Brefeldin A,其中前两者协同激活细胞,后者抑制蛋白转运,从而有效增强胞内细胞因子的积累与检测信号。 03 封闭剂 单核细胞、巨噬细胞和B细胞表面高表达Fc受体,易非特异性结合抗体的Fc段,导致假阳性信号。为解决此问题,菲恩生物提供Purified Anti-Mouse CD16/32 Antibody(FNab30106)用于小鼠样本封闭,;Human Fc Receptor Blocking reagent(K128)用于人样本封闭。关键在于必须选择与样本
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