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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
-20℃ to -80℃
- 保质期:
12个月
- 英文名:
Recombinant Human IL1R2 / IL1RB / CD121b Protein (ECD, His Tag)
- 库存:
99
- 供应商:
北京义翘神州科技股份有限公司
- 规格:
1.00 mg/50.00 µg/100.00 µg
| 规格: | 1.00 mg | 产品价格: | ¥18400.0 |
|---|---|---|---|
| 规格: | 50.00 µg | 产品价格: | ¥2570.0 |
| 规格: | 100.00 µg | 产品价格: | ¥3220.0 |
蛋白名称:Human IL1R2 / IL1RB / CD121b Protein (ECD, His Tag)
蛋白构建:A DNA sequence encoding the extracellular domain (Met 1-Glu 343) of human IL1R2 (NP_004624.1) was expressed, with a fused C-terminal polyhistidine tag.
表达宿主:HEK293 Cells
蛋白纯度:> 98 % as determined by SDS-PAGE
蛋白活性:Measured by its ability to inhibit Interferon gamma secretion by human natural killer lymphoma NK-92 cells in the presence of 500pg/mL IL1b. The EC50 for this effect is typically 20-80ng/mL.
蛋白内毒素:< 1.0 EU per μg of the protein as determined by the LAL method
预测N端:Phe 14
蛋白分子量:The recombinant human IL1R2 consists of 341 amino acids and has a predicted molecular mass of 39.2 kDa. As a result of glycosylation, the rh IL1R2 migrates as an approximately 50-55 kDa band in SDS-PAGE under reducing conditions.
蛋白NP号:NP_004624.1
蛋白氨基酸序列:Met1-Glu343
蛋白标签:C-His
蛋白保存条件:Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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文献和实验2, Arnouk, SM; et al. Depleting IL1R2+ Tumor-Infiltrating Regulatory T Cells with an ADCC-Prone Nanobody Construct Boosts the Efficacy of Anti-PD1 Immunotherapy. Cancer research, PMID: 40960523
, TNFSF5, TNFSF6, TNFSF11, VEGF. Receptors: CD3E, CD3G, CD3Z, CD69, CNR1, CSF1R, CSF2RB, EDG1, EGFR, EPOR, FCER1A, FCER2, FCGR1A, FCGR3A, IFNAR1, IFNAR2, IFNGR1, IFNGR2, IL1R1, IL1R2, IL2RA, IL2RG, IL4R, IL6ST, IL8RA, IL10RA, IL10RB, IL20RA, IL
,2F12;CRIS-3 Leu LFA-1(gp180/95)的α链 与ICAM-1(CD54)和ICAM-2(CD102)结合 CD11b Mol,OKM1; (Mac-1,CR3,integrin αm G,M,NK gp165/95的α链 C3bi和FB受体,与ICAM-1结合,粘附,调理吞噬 CD11c LeuM5;(CR4,integrin αx) M,G,NK,Tsub gp150/95的α链 C3bi受体,调理吞噬
x 106/ml; 3.4 加入1 000 U/ml 的重组人IFN-g培养; 3.5 24h 后加入50ng/ml 的CD3 单克隆抗体和300 U/ml 的重组人IL-2,刺激CIK 细胞的生长和增殖; 注:此时也可同时加入100 U/ml的重组人IL-1a。 3.6 每3天半量换液或扩瓶一次,并补加重组人IL-2 300 U/ml; 3.7 在培养的第7d,收获CIK细胞,此时数量应达到1x 109个以上。 3.8 CIK细胞质控: 3.8.1 台盼蓝染色检测
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