carbonic anhydrase XII/CA12重组蛋白|Recombinant Human Carbonic Anhydrase XII / CA12 Protein (His Tag)
- ￥1790 - 29480
- Sino Biological
- 北京
- 10617-H08H
- 2025年08月12日
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- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
-20℃ to -80℃
- 保质期：
12个月
- 英文名：
Recombinant Human Carbonic Anhydrase XII / CA12 Protein (His Tag)
- 库存：
99
- 供应商：
北京义翘神州科技股份有限公司
- 规格：
1.00 mg/10.00 µg/20.00 µg/50.00 µg
| 规格： | 1.00 mg | 产品价格： | ￥29480.0 |
|---|---|---|---|
| 规格： | 10.00 µg | 产品价格： | ￥1790.0 |
| 规格： | 20.00 µg | 产品价格： | ￥3220.0 |
| 规格： | 50.00 µg | 产品价格： | ￥3220.0 |
蛋白名称：Human Carbonic Anhydrase XII / CA12 Protein (His Tag)
蛋白构建：A DNA sequence encoding the extracellular domain of human CA12 (NP_001209.1) (Met 1-Gln 291) was expressed with a C-terminal polyhistidine tag.
表达宿主：HEK293 Cells
蛋白纯度：> 97 % as determined by SDS-PAGE
蛋白活性：Measured by its esterase activity. The specific activity is >40 pmoles/min/μg, as measured with 1 mM 4-Nitrophenyl acetate and 2.5 μg enzyme at 400 nm in 100 μL of 12.5 mM Tris, 75 mM NaCl, pH 7.5.
蛋白内毒素：< 1.0 EU per μg of the protein as determined by the LAL method
预测N端：Ala 25
蛋白分子量：The recombinant human CA12 consisting of 278 amino acids and has a calculated molecular mass of 31.6 kDa. As a result of glycosylation, the recombinant protein migrates as an approximately 40-45 kDa protein in SDS-PAGE under reducing conditions.
蛋白NP号：NP_001209.1
蛋白氨基酸序列：Met1-Gln291
蛋白标签：C-His
蛋白保存条件：Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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文献和实验碳酸酐酶 carbonic anhydrase carbonatehydrolyase
为催化 H2 CO3 CO2 ＋ H2 O反应的酶，是锌蛋白质（动物原性）， EC 4． 2． 1． 1，存在于脊椎动物的红血球和许多动物的各种组织以及植物的叶中。它在红血球中具有对碳酸和重碳酸离子的迅速转换的作用。在胃中对盐酸的分泌起作用，一般来说，具有调节体液 pH的作用。另外认为与植物的光合成有关系。
【Jounal club】NATURE MEDICINE: 利用蛋白组技术发现糖尿病眼底病变新机制
spectroscopy–based proteomics, we detected 117 proteins in human vitreous and elevated levels of extracellular carbonic anhydrase-I (CA-I) in vitreous from individuals with diabetic retinopathy, suggesting that retinal hemorrhage and erythrocyte lysis
The Application of the Chemical Array for Biological Study
library GLORIA (Gene Library of Osada Laboratory at RIKEN for chemical array analysis) has been established. Using the systematic platform, we detected novel inhibitors of carbonic anhydrase II. We also have shown that this screening method is useful
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