- ￥1790 - 29480
- Sino Biological
- 北京
- 10617-H08H
- 2026年01月26日
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- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
-20℃ to -80℃
- 保质期：
12个月
- 英文名：
Recombinant Human Carbonic Anhydrase XII / CA12 Protein (His Tag)
- 库存：
99
- 供应商：
北京义翘神州科技股份有限公司
- 规格：
1.00 mg/10.00 µg/20.00 µg/50.00 µg
| 规格： | 1.00 mg | 产品价格： | ￥29480.0 |
|---|---|---|---|
| 规格： | 10.00 µg | 产品价格： | ￥1790.0 |
| 规格： | 20.00 µg | 产品价格： | ￥3220.0 |
| 规格： | 50.00 µg | 产品价格： | ￥3220.0 |
蛋白名称：Human Carbonic Anhydrase XII / CA12 Protein (His Tag)
蛋白构建：A DNA sequence encoding the extracellular domain of human CA12 (NP_001209.1) (Met 1-Gln 291) was expressed with a C-terminal polyhistidine tag.
表达宿主：HEK293 Cells
蛋白纯度：> 97 % as determined by SDS-PAGE
蛋白活性：Measured by its esterase activity. The specific activity is >40 pmoles/min/μg, as measured with 1 mM 4-Nitrophenyl acetate and 2.5 μg enzyme at 400 nm in 100 μL of 12.5 mM Tris, 75 mM NaCl, pH 7.5.
蛋白内毒素：< 1.0 EU per μg of the protein as determined by the LAL method
预测N端：Ala 25
蛋白分子量：The recombinant human CA12 consisting of 278 amino acids and has a calculated molecular mass of 31.6 kDa. As a result of glycosylation, the recombinant protein migrates as an approximately 40-45 kDa protein in SDS-PAGE under reducing conditions.
蛋白NP号：NP_001209.1
蛋白氨基酸序列：Met1-Gln291
蛋白标签：C-His
蛋白保存条件：Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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文献和实验2, Torres, SW; et al. Molecular Determinants of Affinity and Isoform Selectivity in Protein─Small Molecule Hybrid Inhibitors of Carbonic Anhydrase. Bioconjugate chemistry, PMID: 40030409
components, and other miscellaneous glycoproteins. In the kidney, several GPI-anchored proteins have been identified, including uromodulin (Tamm-Horsfall glycoprotein), carbonic anhydrase type IV, alkaline phosphatase, Thy-1, BP-3, aminopeptidase P
carbonic anhydrase B, J. Biol. Chem. 267 (1992) 13327-13334. 51. X.D. Geng, X.Q. Chang, High performance hydrophobic interaction chromatography as a tool for protein refolding, J. Chromatogr. A 599 (1992) 185-194. 52. J.D. Carlson, M.L. Yarmush
【资源】全面归类网址：毕赤酵母表达各种来源蛋白（细菌，真菌，植物，人类等）
Porcine follicle-stimulating hormoneS, 10 mg/L, PHO1[174]Porcine inhibitor of carbonic anhydrase (transferrin family)S, 5 mg/L, α-MF[175]Porcine single-chain interleukin-12S, 4 mg/L,α-MF, active370Porcine leukocyte 12-lipoxygenaseI[176]Porcine lactoferrinS
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