- ￥2570 - 4520
- Sino Biological
- 北京
- 10688-HNCH
- 2025年08月12日
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- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
-20℃ to -80℃
- 保质期：
12个月
- 英文名：
Recombinant Human DPP4 / DPPIV / CD26 Protein
- 库存：
99
- 供应商：
北京义翘神州科技股份有限公司
- 规格：
10.00 µg/20.00 µg/50.00 µg
| 规格： | 10.00 µg | 产品价格： | ￥2570.0 |
|---|---|---|---|
| 规格： | 20.00 µg | 产品价格： | ￥4520.0 |
| 规格： | 50.00 µg | 产品价格： | ￥4520.0 |
蛋白名称：Human DPP4 / DPPIV / CD26 Protein
蛋白构建：The native mature form of human DPPIV (NP_001926.2) extracellular domain (Asn 29-Pro 766) was expressed and purified.
表达宿主：HEK293 Cells
蛋白纯度：> 70 % as determined by SDS-PAGE
蛋白活性：1. Measured by its ability to cleave the fluorogenic peptide substrate, Gly-Pro-7-amido-4-methylcoumarin (GP-AMC). The specific activity is > 2,500 pmoles/min/μg. 2. Using the Octet RED System, the affinity constant (Kd) of human DPP4 (Cat: 10688-HNCH) bound to Spike (HCoV-EMC/2012) (Cat: 40071-V31B) was 20 nM. 3. Using the Octet RED System, the affinity constant (Kd) of human DPP4 (Cat: 10688-HNCH) bound to Spike (HCoV-EMC/2012) (Cat: 40071-V05B) was 50 nM. 4. Using the Octet RED System, the affinity constant (Kd) of human DPP4 (Cat: 10688-HNCH) bound to Spike (HCoV-EMC/2012) (ECD, aa 1-1297) (Cat: 40069-V08B) was 33 nM. 5. Using the Octet RED System, the affinity constant (Kd) of human DPP4 (Cat: 10688-HNCH) bound to Spike-His (aa 1-760) (Cat: 40021-V08H) was 12 nM.
蛋白内毒素：< 1.0 EU per μg of the protein as determined by the LAL method
预测N端：Asn 29
蛋白分子量：The recombinant human DPPIV exists as the mature active form, and consists of 738 amino acids with a predicted molecular mass of 85.4 kDa. The apparent molecular mass of the glycosylated rhDPPIV is approximately 95 kDa in SDS-PAGE under reducing conditions.
蛋白NP号：NP_001926.2
蛋白氨基酸序列：Asn29-Pro766
蛋白标签：N-cleavage
蛋白保存条件：Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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文献和实验2, Zhang H, et al. Oral glucagon-like peptide 1 analogue ameliorates glucose intolerance in db/db mice.Biotechnology letters, PubMed ID: 36006576
3, Kan R, et al. Identification and molecular docking study of sugarcane leaves-derived compounds as potent dipeptidyl peptidase IV, α-glucosidase, and α-amylase inhibitors.Journal of the science of food and agriculture, PubMed ID: 37038045
Using the scan‐x Web Site to Predict Protein Post‐Translational Modifications
spectrometry data is providing evidence that almost every protein in the cell undergoes some form of post?translational modification. We describe a protocol to use the scan?x Web site to view predicted acetylation sites in the human proteome and predicted
Clonality - X Chromosome Inactivation Assay
of HpaII and HhaI sites near the polymorphic CAG repeat in the human androgen-receptor gene correlates with X chromosome inactivation. Am J Hum Genet 51:1229-39, 1992 . Enomoto T, Fujita M, Inoue M, Tanzawa O, Nomura T, Shroyer K. Analysis
Clonality - X Chromosome Inactivation Assay
of HpaII and HhaI sites near the polymorphic CAG repeat in the human androgen-receptor gene correlates with X chromosome inactivation. Am J Hum Genet 51:1229-39, 1992 . Enomoto T, Fujita M, Inoue M, Tanzawa O, Nomura T, Shroyer K. Analysis
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