- ￥1790 - 14950
- Sino Biological
- 北京
- 10440-H08H
- 2026年01月26日
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- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
-20℃ to -80℃
- 保质期：
12个月
- 英文名：
Recombinant Human Alkaline Phosphatase / ALPL Protein (His Tag)
- 库存：
99
- 供应商：
北京义翘神州科技股份有限公司
- 规格：
10.00 µg/20.00 µg/50.00 µg/500.00 µg
| 规格： | 10.00 µg | 产品价格： | ￥1790.0 |
|---|---|---|---|
| 规格： | 20.00 µg | 产品价格： | ￥3220.0 |
| 规格： | 50.00 µg | 产品价格： | ￥3870.0 |
| 规格： | 500.00 µg | 产品价格： | ￥14950.0 |
蛋白名称：Human Alkaline Phosphatase / ALPL Protein (His Tag)
蛋白构建：A DNA sequence encoding the human tissue-nonspecific alkaline phosphatase (NP_000469.3) (Met 1-Ser 502) was expressed with a C-terminal polyhistidine tag.
表达宿主：HEK293 Cells
蛋白纯度：> 95 % as determined by SDS-PAGE
蛋白活性：Measured by its ability to cleave a fluorogenic substrate, 4-Methylumbelliferyl phosphate (4-MUP). The specific activity is > 50, 000 pmoles/min/μg.
蛋白内毒素：< 1.0 EU per μg of the protein as determined by the LAL method
预测N端：Leu 18
蛋白分子量：The recombinant human ALPL consists of 496 amino acids and predicts a molecular mass of 55 kDa. As a result of glycosylation, rhALPL migrates as approximately 65 kDa band in SDS-PAGE under reducing conditions.
蛋白NP号：NP_000469.3
蛋白氨基酸序列：Met1-Ser502
蛋白标签：C-His
蛋白保存条件：Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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文献和实验2, Moyer, TC; et al. Highly conserved brain vascular receptor ALPL mediates transport of engineered AAV vectors across the blood-brain barrier. Molecular therapy : the journal of the American Society of Gene Therapy, PMID: 40340250
HIS 标签蛋白纯化效果不理想，宝宝心里苦呀。今天，小编来跟大家一起找找原因。 纯化所得组分中没有收集到重组的 HIS 标签蛋白。该问题主要可以分为以下两个方面： 1、HIS 标签蛋白没有结合到填料上就流穿了 原因一 超声的功率不对。超声功率过高，容易导致蛋白炭化；功率过低，蛋白释放不出来。 建议 改变超声功率，同时可以在超声前加入溶菌酶。 原因二 结合缓冲液条件不合适。 建议 检查结合缓冲液中是否有影响结合的因素，如：金属离子螫合剂、强还原剂、过高的咪唑浓度。同时，优化缓冲
Immunostaining with Anti-Digoxigenin Alkaline Phosphatase
Materials Anti-digoxigenin-AP conjugate (Roche #11093274910, 150U/200 uL, $168) Levamisole (Sigma #T1512, 2 g, $10) (blocks endogenous phosphatase activity) BCIP (Roche #11383221001, 3 mL, $49) NBT (Roche #11383213001, 3 mL, $48)
DETECTION OF ß-GALACTOSIDASE AND ALKALINE PHOSPHATASE ACTIVITIES IN TISSUE
of X-gal can be easier to detect prior to carrying out the X-Phos reaction as background alkaline phosphatase staining can obscure it, particularly in whole mounts. Rinse the tissue very well with PBS prior to PLAP staining as residual ß-gal activity
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