Alkaline Phosphatase/ALPL重组蛋白|Recombinant Human Alkaline Phosphatase / ALPL Protein (His Tag)
- ￥1790 - 14950
- Sino Biological
- 北京
- 10440-H08H
- 2025年08月12日
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- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
-20℃ to -80℃
- 保质期：
12个月
- 英文名：
Recombinant Human Alkaline Phosphatase / ALPL Protein (His Tag)
- 库存：
99
- 供应商：
北京义翘神州科技股份有限公司
- 规格：
10.00 µg/20.00 µg/50.00 µg/500.00 µg
| 规格： | 10.00 µg | 产品价格： | ￥1790.0 |
|---|---|---|---|
| 规格： | 20.00 µg | 产品价格： | ￥3220.0 |
| 规格： | 50.00 µg | 产品价格： | ￥3870.0 |
| 规格： | 500.00 µg | 产品价格： | ￥14950.0 |
蛋白名称：Human Alkaline Phosphatase / ALPL Protein (His Tag)
蛋白构建：A DNA sequence encoding the human tissue-nonspecific alkaline phosphatase (NP_000469.3) (Met 1-Ser 502) was expressed with a C-terminal polyhistidine tag.
表达宿主：HEK293 Cells
蛋白纯度：> 95 % as determined by SDS-PAGE
蛋白活性：Measured by its ability to cleave a fluorogenic substrate, 4-Methylumbelliferyl phosphate (4-MUP). The specific activity is > 50, 000 pmoles/min/μg.
蛋白内毒素：< 1.0 EU per μg of the protein as determined by the LAL method
预测N端：Leu 18
蛋白分子量：The recombinant human ALPL consists of 496 amino acids and predicts a molecular mass of 55 kDa. As a result of glycosylation, rhALPL migrates as approximately 65 kDa band in SDS-PAGE under reducing conditions.
蛋白NP号：NP_000469.3
蛋白氨基酸序列：Met1-Ser502
蛋白标签：C-His
蛋白保存条件：Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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文献和实验in solution c. for 5-15 minutes at room temperature. Wash, counterstain in haematoxylin, wash. Mount in phosphate buffered glycerine jelly RESULTS Alkaline phosphatase activity - Red Nuclei - Blue
DETECTION OF ß-GALACTOSIDASE AND ALKALINE PHOSPHATASE ACTIVITIES IN TISSUE
salt NBT, (Altman, 1972) can be used in conjunction with X-Phos as the final electron acceptor for the indoxyl dimerization reaction. When reduced, NBT forms a purple precipitate. Of the cloned phosphatases, the human placental alkaline
Human Placental Alkaline Phosphatase Stain
The following protocol is for detection of human placental alkaline phosphatase (AP) in cultured cells. Human placental AP is heat stable, unlike other AP proteins typically expressed by cells. A few cell types (HeLa cells, for example
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