分析内容 1. 数据产出统计:对测序结果进行图像识别、去污染、去接头,统计结果包括read长度、read数据、数据产量;
2. RRBS序列与参考序列的比对;
3. Promoter和CpG岛(CGI)覆盖度分析;
4. Promoter和CGI区甲基化分析;
5. 差异性甲基化区域(DMR)分析。
参考文献 [1] Wang L, Sun J, Wu H, et al. Systematic assessment of reduced representation bisulfite sequencing to human blood samples: A promising method for large-sample-scale epigenomic studies. J Biotechnol. 2012.
[2] Li S, chowdhry, Liu F et al. Tumor-Suppressive miR148a Is Silenced by CpG Island Hypermethylation in IDH1-Mutant Gliomas. Clin Cancer Res. 2014.
[3] Terragni J, Zhang G, Sun Z, et al. Notch signaling genes: myogenic DNA hypomethylation and 5-hydroxymethylcytosine. Epigenetics, 2014.