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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
在-20℃ to -80℃
- 保质期:
12个月
- 英文名:
Recombinant Human Podoplanin / PDPN Protein (ECD, His & Fc Tag)
- 库存:
99
- 供应商:
北京义翘神州科技股份有限公司
- 规格:
100 µg
蛋白名称:Podoplanin蛋白, Podoplanin protein
蛋白构建:A DNA sequence encoding the extracellular domain of human PDPN short isoform (Q86YL7-1) (Met 1-Lys 123) was fused with the C-terminal polyhistidine-tagged Fc region of human IgG1 at the C-terminus.
表达宿主:HEK293 Cells
蛋白纯度:> 95 % as determined by SDS-PAGE
蛋白活性:1. Measured by its binding ability in a functional ELISA. 2. Immobilized human Podoplanin (Cat: 11065-H03H) at 10 μg/mL (100 μl/well) can bind biotinylated human CLEC1B-His (Cat:10976-H07H), The EC50 of biotinylated human CLEC1B-His is 0.4-1.0 μg/mL.
蛋白内毒素:< 1.0 EU per μg of the protein as determined by the LAL method
预测N端:Glu 21
蛋白分子量:The recombinant human PDPN/Fc chimera is a disulfide-linked homodimer. The reduced monomer comprises 351 amino acids with a predicted molecular mass of 38.6 kDa. As a result of glycosylation, rh PDPN/Fc monomer migrates as an approximately 50-55 kDa band in SDS-PAGE under reducing conditions.
蛋白NP号:Q86YL7-1
蛋白氨基酸序列:Met1-Lys123
蛋白标签:C-human IgG1-Fc & His
蛋白保存条件:Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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文献和实验High Throughput Production of Recombinant Human Proteins for Crystallography
, to be applied based on specific knowledge of protein families or when the core protocol is unsatisfactory. This scheme has been applied to more than 550 human proteins (x003E;10,000 constructs) and has resulted in the deposition of 112 unique structures
Preparation of Recombinant Protein Spotted Arrays for Proteome‐Wide Identification of Kinase Targets
, H. 2009. Profiling the human protein‐DNA interactome reveals ERK2 as a transcriptional repressor of interferon signaling. Cell 139:610‐622. Hudson, J.R., Jr., Dawson, E.P
Protein Microarrays for Identification of Novel Extracellular Protein‐Protein Interactions
microbead complexes. (A ) Schematic model of a protein A‐microbead complex with extracellular domain (ECD)‐Fc fusion protein. Protein A is shown attached to the microbead and binding the Fc (blue) domain of the captured bait protein. (B ) Schematic model
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