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- Sino Biological
- 10149-H02H
- 2025年08月12日
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- 详细信息
- 文献和实验
- 技术资料
- 规格：
50 µg
蛋白名称：Endoglin/CD105蛋白, Endoglin/CD105 protein
蛋白构建：A DNA sequence encoding the extracellular domain (Met 1-Gly 586) of human CD105 (NP_001108225.1) precursor was expressed with C-terminal fused human IgG1 Fc region.
表达宿主：HEK293 Cells
蛋白纯度：> 95 % as determined by SDS-PAGE
蛋白活性：1. Measured by its ability to bind biotinylated Human TGFRB1 in a functional ELISA. 2. Measured by its ability to bind biotinylated Human ALK1-Fc (Cat:10066-H02H) in functional Elisa.
蛋白内毒素：< 1.0 EU per μg of the protein as determined by the LAL method
预测N端：Glu 26
蛋白分子量：The recombinant human CD105/Fc chimera is a disulfide-linked homodimeric protein. The reduced monomer consists of 799 amino acids and has a predicted molecular mass of 87.5 kDa. As a result of glycosylation, rhCD105/Fc monomer migrates as an approximately 115-120 kDa protein in SDS-PAGE under reducing conditions.
蛋白NP号：NP_001108225.1
蛋白氨基酸序列：Met1-Gly586
蛋白标签：C-human IgG1-Fc
蛋白保存条件：Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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文献和实验Purification of Adipose Tissue Mesenchymal Stem Cells and Differentiation Toward Hepatic-Like Cells
yet; therefore, it is still not obvious how to purify these heterogeneous fraction of cells. We postulate that one of the markers defining MSC provenance is CD105 (endoglin). Therefore, we have sorted CD105+ fraction of AT-MSCs, expanded them, and differentiated
Barry FP, et al. The monoclonal antibody SH-2, raised against human mesenchymal stem cells, recognizes an epitope on endoglin (CD105). Biochem.Biophys.Res
蛋白质纯化的原理和方法（Protein Purification Principles and Methods）
and Hydrophobic-Interactions •“Weak” interactions are important for structure and function (activity) of the protein ÆIn most cases the purification must be gentle! Before the purification… •Cultivation of bacteria •Cell disruption
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