- ￥3220 - 21030
- Sino Biological
- 北京
- 10624-H08B
- 2025年08月12日
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- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
在-20℃ to -80℃
- 保质期：
12个月
- 英文名：
Recombinant Human CDK2 / p33 Protein (His Tag)
- 库存：
99
- 供应商：
北京义翘神州科技股份有限公司
- 规格：
50 µg/500 µg
| 规格： | 50 µg | 产品价格： | ￥3220.0 |
|---|---|---|---|
| 规格： | 500 µg | 产品价格： | ￥21030.0 |
蛋白名称：Human CDK2 / p33 Protein (His Tag)
蛋白构建：A DNA sequence encoding the human cyclin-dependent kinase 2 isoform 1 (NP_001789.2) (Met 1-Leu 298) was expressed, fused with a polyhistidine tag at the C-terminus.
表达宿主：Baculovirus-Insect Cells
蛋白纯度：> 85 % as determined by SDS-PAGE
蛋白活性：No Kinase Activity
蛋白内毒素：< 1.0 EU per μg of the protein as determined by the LAL method
预测N端：Met
蛋白分子量：The recombinant human CDK2 consists of 308 amino acids and predicts a molecular mass of 35 kDa. The apparent molecular mass of rhCDK2 is approximately 33 kDa in SDS-PAGE under reducing conditions.
蛋白NP号：NP_001789.2
蛋白氨基酸序列：Met1-Leu298
蛋白标签：C-His
蛋白保存条件：Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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文献和实验. Natl. Acad. Sci. U. S. A. 98, 12438–12443 [57] Gius, D.R. et al. (1999) Transduced p16INK4a peptides inhibit hypophosphorylation of the retinoblastoma protein and cell cycle progression prior to activation of Cdk2 complexes in late G1. Cancer Res. 59
v468.Chapter 3 分泌型跨膜相关蛋白提纯及Wnt抑制活性
serumfreeconditioned medium (11) ). 3. Methods When expressing recombinant proteins, the choice of vector hasa major impact on purification strategy, yield, and utility of theproduct. Vectors that link a poly-histidine tag to the recombinant protein typically
, lilie H, et al. Folding proteins. In:Creighton T E ed. Protein Function: A Practical Approach, 2nd.New York: IRL,1997,57-99 [7]CowleyDT, MackinRB. Expression, purification and charaterization of recombinant human proinsulin. FEBS Lett,1997,402:124
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