- ￥2570 - 3220
- Sino Biological
- 北京
- 10044-H07B
- 2025年08月12日
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- 详细信息
- 询价记录
- 文献和实验
- 技术资料
- 保存条件：
-20℃ to -80℃
- 保质期：
12个月
- 英文名：
Recombinant Human GSK3B Protein (His Tag)
- 库存：
99
- 供应商：
北京义翘神州科技股份有限公司
- 规格：
20.00 µg/50.00 µg
| 规格： | 20.00 µg | 产品价格： | ￥2570.0 |
|---|---|---|---|
| 规格： | 50.00 µg | 产品价格： | ￥3220.0 |
蛋白名称：Human GSK3B Protein (His Tag)
蛋白构建：The amino acids corresponding to the full length of human GSK3B isoform 1 (NP_002084.2) (Met 1-Thr 433) was fused with a polyhistidine tag at the N-terminus.
表达宿主：Baculovirus-Insect Cells
蛋白纯度：> 90 % as determined by SDS-PAGE
蛋白活性：1. The specific activity was determined to be 45 nmol/min/mg using synthetic Phospho-Glycogen Synthase Peptide-2 (YRRAAVPPSPSLSRHSSPHQpSEDEEE) as substrate. 2. Immobilized His-GSK3B at 10 μg/ml (100 μl/well) can bind biotinylated human HG3C-CTNNB1 (cat:11279-H20B), EC50 of biotinylated human HG3C-CTNNB1 (cat:11279-H20B) is 0.15-0.35 μg/ml.
蛋白内毒素：< 1.0 EU per μg of the protein as determined by the LAL method
预测N端：Met
蛋白分子量：The recombinant human GSK3B consists of 452 amino acids and predicts a molecular mass of 50.4 kDa. The apparent molecular mass of rhGSK3B is approximately 44-48 kDa in SDS-PAGE under reducing conditions.
蛋白NP号：NP_002084.2
蛋白氨基酸序列：Met1-Thr433
蛋白标签：N-His
蛋白保存条件：Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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文献和实验2, Gao J, et al. Icariside II, a phosphodiesterase 5 inhibitor, attenuates cerebral ischemia/reperfusion injury by inhibiting GSK-3β-mediated activation of autophagy.British journal of pharmacology, PubMed ID: 31658364
3, Yue S, et al. Highly conserved shifts in ubiquitin-proteasome system (UPS) activity drive mitochondrial remodeling during quiescence.Nature communications, PubMed ID: 35915093
4, Zhu S, et al. CDK1 bridges NF-κB and β-catenin signaling in response to H. pylori infection in gastric tumorigenesis.Cell reports, PubMed ID: 36681899
5, He DL, et al. Identification of AS1842856 as a novel small-molecule GSK3α/β inhibitor against Tauopathy by accelerating GSK3α/β exocytosis.Aging cell, PubMed ID: 39287420
v468. chapter 4 WNT 细胞通路 检测细胞GSK3活性与表达水平
organelles. Two isoforms areencoded by separate genes; GSK3b (chromosome 19q13.2)encodes a protein of 51 kDa, while GSK3b (chromosome 3q13.3)encodes a protein of 47 kDa (1 – 3) . The difference in size is dueto a glycine-rich N-terminal insertion in GSK
Identifying Protein Interactions by Hydroxyl‐Radical Protein Footprinting
Basic Protocol 1: Generation of an Active Recombinant Protein That is End‐Labeled Basic Protocol 2: Hydroxyl Radical Cleavage of Protein in Absence and Presence of Ligand Basic Protocol 3: Analyze Gel
Strategies to Optimize Protein Expression in E. coli
Peng, L., Xu, Z.N., Fang, X.M., Wang, F., Yang, S., and Cen, P.L. 2004. Preferential codons enhancing the expression level of human beta‐defensin‐2 in recombinant Escherichia coli. Protein Pept. Lett. 11:339
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