重组人 GSK3B 蛋白 (His标签)(产品说明)
蛋白名称:Human GSK3B Protein (His Tag)
蛋白构建:The amino acids corresponding to the full length of human GSK3B isoform 1 (NP_002084.2) (Met 1-Thr 433) was fused with a polyhistidine tag at the N-terminus.
表达宿主:Baculovirus-Insect Cells
蛋白纯度:> 90 % as determined by SDS-PAGE
蛋白活性:1. The specific activity was determined to be 45 nmol/min/mg using synthetic Phospho-Glycogen Synthase Peptide-2 (YRRAAVPPSPSLSRHSSPHQpSEDEEE) as substrate.2. Immobilized His-GSK3B at 10 μg/ml (100 μl/well) can bind biotinylated human HG3C-CTNNB1 (cat:11279-H20B), EC50 of biotinylated human HG3C-CTNNB1 (cat:11279-H20B) is 0.15-0.35 μg/ml.
蛋白内毒素:< 1.0 EU per μg of the protein as determined by the LAL method
预测N端:Met
蛋白分子量:The recombinant human GSK3B consists of 452 amino acids and predicts a molecular mass of 50.4 kDa. The apparent molecular mass of rhGSK3B is approximately 44-48 kDa in SDS-PAGE under reducing conditions.
蛋白NP号:NP_002084.2
蛋白氨基酸序列:Met1-Thr433
蛋白标签:N-His
蛋白保存条件:Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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4, Gao, J; et al. Icariside II, a phosphodiesterase 5 inhibitor, attenuates cerebral ischemia/reperfusion injury by inhibiting GSK-3β-mediated activation of autophagy. Br. J. Pharmacol., Pubmed: 31658364