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- Thermo scientific -fermentas
- ER0981
- 2025年10月08日
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|
| Catalog# | Size, concentration | Supplied with: | Certificate of Analysis | MSDS | |
| 10X Buffer R | 10X Buffer Tango™ | ||||
| ER0981 | 300 u (10 u/µl) | 1.00 ml | 1.00 ml | ER0981 | |
| ER0982 | 1500 u (10 u/µl) | 1.00 ml | 1.00 ml | ER0982 | |
| ER0983 | HC, 1500 u (50 u/µl) | 1.00 ml | 1.00 ml | ER0983 | |
| Recommended buffer for 100% activity | Optimal temperature | Enzyme activity in Fermentas buffers, % | Tango™ buffer for double digestion | |||||
|---|---|---|---|---|---|---|---|---|
| B (blue) 1X |
G (green) 1X |
O (orange) 1X |
R (red) 1X |
Tango™ (yellow) 1X / 2X |
||||
| R | 65°C | 50-100 | 50-100 | 20-50 | 100 | 50-100 | 100 | 1X or 2X |
Lambda DNA
1.4%琼脂糖
195 切割位点
10 mM Tris-HCl (pH 8.5, 37°C), 10 mM MgCl2 , 100 mM KCl, 0.1 mg/ml BSA。
65°C酶切。为确保高效酶切，反应体系中加入石蜡油防止蒸发。
10 mM Tris-HCl (pH 7.4, 25°C), 100 mM KCl, 1 mM DTT, 1 mM EDTA, 0.2 mg/ml BSA和50% (v/v)甘油。
备注
Dcm: 无重叠 – 不影响。
CpG: 无重叠 – 不影响。
EcoKI: 可能重叠 – 阻止酶切。
EcoBI: 无重叠 – 不影响。
| Methylation type | Sequence | Cleavage effect |
|---|---|---|
| EcoKI (AAC(N)6 GTGC) | 5'...TTAm6AC(N)6 G TGC...3' |
阻止酶切 |
| bp from the recognition site to fragment end | ||||
|---|---|---|---|---|
| 1 | 2 | 3 | 4 | 5 |
| 0 | 0-20 | 50-100 | ||
| Lambda | ΦX174 | M13mp18/19 | pBR322 | puc18/19 | pUC57 |
|---|---|---|---|---|---|
| 195 | 35 | 63 | 15 | 13 | 13 |
| pTZ19R/U | pTZ57R | pBluescriptIIKS(-/+) | pBluescriptIISK(-/+) | pACYC177 | pACYC184 |
| 18 | 18 | 19 | 19 | 16 | 12 |
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文献和实验AFLP: not only for fingerprinting, but for positional cloning
, one EcoRI (having a 6 bp recognition site) and the other MseI (having a 4 bp recognition site). EcoRI cuts ~ every 2-2.5 Kb in Arabidopsis, while MseI cuts ~ every 300-400 bp. Thus a large number of fragments are generated with relative abundances
AFLP: not only for fingerprinting, but for positional cloning
from each ecotype is digested to completion with two restriction enzymes, one EcoRI (having a 6 bp recognition site) and the other MseI (having a 4 bp recognition site). EcoRI cuts ~ every 2-2.5 Kb in Arabidopsis, while MseI cuts ~ every 300-400 bp. Thus a large
and Landsberg erecta. 1) Genomic DNA from each ecotype is digested to completion with two restriction enzymes, one EcoRI (having a 6 bp recognition site) and the other MseI (having a 4 bp recognition site). EcoRI cuts ~ every 2-2.5 Kb in Arabidopsis, while MseI
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