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- Thermo scientific -fermentas
- FD0194
- 2025年10月01日
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| Catalog# | Size, concentration | Supplied with: | Certificate of Analysis | MSDS | |
| 10X FastDigest® Buffer | 10X FastDigest® Green Buffer | ||||
| FD0194 | 100 µl (100 react.) | 1.00 ml | 1.00 ml | FD0194 | |
| Reaction temperature | Digestion time with 1µl of FastDigest® enzyme, min | bp from end of DNA required for complete digestion | Thermal inactivation | Incubation time without star activity, hours | |||
|---|---|---|---|---|---|---|---|
| Lambda, 1µg/20µl | Plasmid DNA, 1µg/20µl | PCR product, ~0.2µg/30µl | Genomic DNA, 1µg/10µl | ||||
| 37°C | 5 | 5 | 5 | 5 | 2 | No (chloroform extraction) |
16 |
Lambda DNA
1.0%琼脂糖
74 切割位点
1 µl FastDigest® Sau96I (Cfr13I)可在
– 5分钟内酶切1 µg lambda DNA;
– 5分钟内酶切1 µg质粒DNA;
– 5分钟内酶切0.2 µg PCR扩增产物;
– 5分钟内酶切1 µg基因组DNA或30分钟内酶切5 µg基因组DNA。
Dcm: 可能重叠 – 阻止酶切。
CpG: 可能重叠 – 阻止酶切。
EcoKI: 无重叠 – 不影响。
EcoBI: 无重叠 – 不影响。
| Methylation type | Sequence | Cleavage effect |
|---|---|---|
| Dcm (CCWGG) | 5'...GGNCm5CW GG ...3' |
阻止酶切 |
| CpG | 5'...GGNCm5C G ...3' |
阻止酶切 |
| Lambda | ΦX174 | M13mp18/19 | pBR322 | puc18/19 | pUC57 |
|---|---|---|---|---|---|
| 74 | 2 | 4 | 15 | 6 | 8 |
| pTZ19R/U | pTZ57R | pBluescriptIIKS(-/+) | pBluescriptIISK(-/+) | pACYC177 | pACYC184 |
| 6 | 8 | 8 | 8 | 9 | 11 |
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文献和实验E-Z 96® M13 Isolation Vacuum Manifold Protocol
the E-Z 96 DNA Plate back on the manifold and apply the vacuum for another 5 minutes. Repeat the tapping step from step 11 to completely remove any remaining liquid. 13. Optional: Place the E-Z® 96 DNA Plate into a vacuum oven preset at 60°C
E-Z 96® Viral RNA Protocol with Centrifugation
from step 6 (including any precipitate) to each well of the HiBind® RNA plate. 8. Seal the HiBind® RNA plate with new sealing film. Load the HiBind® RNA plate with 2ml 96-well plate into the plate holder, and place the whole assembly into the rotor
M13 Phage Growth and DNA Purification Using 96 Well Microtiter Trays
The growth and purification of M13 DNA from small volume (1.5mL) cultures is a rapid and easily performed procedure (1 ). The samples can be processed in microcentrifuges in disposable polypropylene tubes and yield sufficient, pure single
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