M13 Phage Growth and DNA Purification Using 96 Well Microtiter Trays

The growth and purification of M13 DNA from small volume (1.5mL) cultures is a rapid and easily performed procedure (1 ). The samples can be processed in microcentrifuges in disposable polypropylene tubes and yield sufficient, pure single-stranded DNA (4 μM) for five or more sequencing experiments. Even when several cultures are to be grown and purified simultaneously, up to 100 can be processed to completion in a day. The handling of this number of samples is tedious, however, and much time is spent opening and closing tubes and transferring tubes in and out of microcentrifuges. One hundred small volume phenol extractions and ethanol precipitations tasks even the more dedicated sequencer.