- 询价
- Thermo scientific -fermentas
- SM1343
- 2025年10月20日
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- 文献和实验
- 技术资料
- 库存：
大量
|
| Catalog# | Size, concentration | Supplied with: | Certificate of Analysis | MSDS |
| 6X Orange DNA Loading Dye | ||||
| SM1343 | 500 applic. (0.1 µg/µl) | 2 x 1.00 ml | SM1343 |
- Features
-
- 适合DNA大小鉴定和粗略定量。
- 条带窄。
- 明亮参考条带(红色标记)。
- 即用型包装可直接上样电泳。 室温可稳定保存6个月。
- 配套提供样本DNA上样染料。
其与6X Orange DNA Loading Dye预先混合(含有二甲苯青FF和orange G)。Orange G在1%琼脂糖凝胶中的迁移速率与50 bp条带接近。因此O’GeneRuler™ DNA ladders更适合小片段的DNA分子检测。
Ladders 产品中配套提供6X Orange DNA Loading Dye可用于样本DNA电泳前处理。
-20°C可保存更长时间。
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文献和实验freeshotgun sequencing library from miniprep BAC DNA
, #27-5266-01) Collected ~ 28 μl DNA in EB buffer were used as insert to be ligated directly. Add 20 μl DNA and 8 μl DNA plus 12 μl ddH2 O into two tubes of the Ready-To-Go respectively, and ligate at 15℃ overnight. Prior to electroporation, the ligation
free shotgun sequencing library from miniprep BAC DNA
and evaluate. Excise DNA in the range from 2 - 4kb from the agarose gel with a clean sharp scalpel, and isolate DNA from gel slices using QIAquick gel extraction kit. In the last step, use 30 μl of EB( elution buffer) to elute DNA from column and collect ~ 28 μ
below and the reactions should be loaded to 15%-20% acrylamide urea gels. If making a sequencing ladder for footprinting, use several hundred thousand cpm per reaction if possible. This will give enough sequenced DNA for many gels (typically 5,000 cpm of the sequencing
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