- ¥1980
- SignaGen
- 美国
- SL100568-MEF
- 2026年04月24日
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- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
4°C
- 保质期：
1年
- 英文名：
GenMute™ siRNA Transfection Reagent for MEFs
- 库存：
现货 大量
- 供应商：
美中清水湾生物科技有限公司
- 规格：
毫升
Description:
GenMute™ siRNA Transfection Reagent for MEFs is pre-optimized for transfecting siRNA to Mouse Embryonic Fibroblasts (MEFs). MEFs are required to support the growth of undifferentiated mouse or human ES cells, iPS cells, thus they are also called feeder cells. MEFs are isolated from mouse embryos and are used at their early passages. Before MEFs are used as feeder cells, they must be treated by irradiation or mitomycin C to stop the cells from further dividing.
Refer to the following optimal transfection conditions for maximal silencing on MEFs. GenMute™ reagent, 1.0 ml, is sufficient for ~833 transfections in 24 well plates or ~416 transfections in 6 well plates.
| Summary of Optimal Transfection Conditions: |
|
Storage:
Store at 4 °C. If stored properly, the product is stable for 12 months or longer.
Data Sheet & Protocol .pdf
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文献和实验Transfection of siRNA oligos to peritoneal macrophages
Use DeliverX Transfection from Panomics Modified protocol below Step 1: Make complexes Note: Do not scale up more than 3x Thaw siRNA and DeliverX reagent Dilute siRNA into buffer 1 10 uM siRNA 2.17 ul Buffer
the 3 hour incubation Dynabeads™ M-280 Streptaviden magnetic beads (7x107 beads, ~100µl/sample) are added to the respective samples (note 1);5.The resultant siRNA/Flag complexes are then eluted with magnetic bead binding, the solution can be discarded
transfection procedures/reagents, DEAE dextran, calcium phosphate and Effectene™. Aims You will work individually, to perform your transfections Ð two people will work at the same time in the tissue culture facilities in the Wolfson building.
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