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| Catalog# | Size, concentration | Certificate of Analysis | MSDS |
| R0601 | 30 ml | R0601 | |
| R0603 | 5 x 1 ml | R0603 |
- Features
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- 无内切或外切脱氧核糖核酸酶、核糖核酸酶、磷酸酶污染。
- 体外转录分析检测。
- 质量检测表明不含内切或外切脱氧核糖核酸酶、核糖核酸酶、磷酸酶污染。
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文献和实验How to make DEPC-treated water and Tris Buffer
Add 0.1 ml DEPC to 100 ml of the solution to be treated and shake vigorously to bring the DEPC into solution.Let the solution incubate for 12 hours at 37℃.Autoclave for 15 minutes to remove any trace of DEPC.Notes:How to make DEPC-treated Tris
Add 0.1% diethylpyrocarbonate (DEPC) to water, mix overnight, and then autoclave 20 min to destroy DEPC
, use DEPC-treated water to generate the solution. Although H2 O prepared with reverse osmosis systems like Milli-Q is free of RNases (Huang 1995) , it can be contaminated by microbial growth if the machine and piping are not well maintained
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