- 询价
- Thermo scientific -fermentas
- R0891
- 2025年10月16日
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| Catalog# | Size, concentration | Supplied with: | Certificate of Analysis | MSDS | |
| 5X Protein Loading Buffer | 20X Reducing Agent (2 M DTT) | ||||
| R0891 | for 2000 samples of 50 µl | 20.00 ml | 4 x 1.50 ml | R0891 | |
- Applications
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- SDS-聚丙烯酰胺凝胶(SDS-PAGE)电泳前蛋白样本处理。
- 5X Loading Buffer: 0.313 M Tris-HCl (pH 6.8, 25°C), 10% SDS, 0.05%溴酚蓝和50%甘油。
- 20X Reducing Agent: 2 M DTT.
20X Reducing Agent需-20°C保存。
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文献和实验【求助】提质粒后，跑电泳，用10乘Loading Buffer 还是6乘Loading Buffer？
ilovelc999 请问各位，提质粒后，用20微升TE溶解，然后进行电泳，应该用10乘Loading Buffer 还是用6乘Loading Buffer？另外Buffer与质粒溶液一般应以什么比例混合好呢？多谢各位啦！ 纯属菜鸟 10乘 5：1的比例 质粒5 肥宝 我是用6X的，1:5，质粒5，不过也没那么严格，一般也就点个小点估计1ul lihuijin017
Jacobs:Protocol Total Protein Isolation Using RIPA Lysis Buffer
Materials RIPA buffer (RIPA buffer enables the extraction of cytoplasmic, membrane and nuclear proteins and is compatible with many applications, including reporter assays, protein assays, immunoassays and protein purification. RIPA
Radiolabeled sequencing gel preparation, loading, and electrophoresis
to the buffer chambers. The wells are cleaned by circulating buffer into the wells with a syringe and, immediately prior to the loading of each sample, the urea in each well is suctioned out with a mouth pipette. Each base-specific sequencing reaction
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