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- Thermo scientific -fermentas
- B47
- 2025年10月15日
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- 文献和实验
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|
| Catalog# | Size, concentration | Certificate of Analysis | MSDS |
| B47 | 1 L | B47 |
- Applications
-
- 天然蛋白聚丙烯酰胺凝胶电泳。
- Western杂交。
- 25 mM Tris,
- 192 mM glycine, pH 8.3
- 每次电泳需新鲜配置1X 缓冲液。
- 使用前需将浓缩液稀释至1X工作溶液。
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文献和实验10X TBE BUFFER 3 Liters 324 g Tris base 165g Boric acid 120mls 0.5M EDTA (pH 8.0) autoclave for 20 min 2 Liters 216g Tris base 110g Boric acid 80mls 0.5M EDTA (pH 8.0) autoclave
from the bottom of the cassette. 3) In one smooth motion, gently pull the comb out of the cassette. 4) Rinse the sample wells with the appropriate 1X SDS Running Buffer. Invert the gel and shake the gel to remove the buffer. Repeat two more times.
How to make DEPC-treated water and Tris Buffer
first, and then dissolve Tris to make the appropriate buffer. Trace amounts of DEPC will modify purine residues in RNA by carboxymethylation. Carboxymethylated RNA is translated with very low efficiency in cell-free systems. However, its ability to form DNA:RNA or RNA:RNA
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