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- 详细信息
- 询价记录
- 文献和实验
- 技术资料
- 库存:
大量
|
| Catalog# | Size, concentration | Supplied with: | Certificate of Analysis | MSDS |
| 10X Bsm Buffer | ||||
| EP0691 | 1600 u (8 u/µl) | 1.25 ml | EP0691 |
- Features
-
- 嗜热性DNA聚合酶,链置换活性强。
- Applications
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- dsDNA 5‘突出端补平法标记。
- 通过以下方法等温DNA扩增 :
– 环介导等温扩增 (LAMP) (1, 2),
– 全基因组扩增 (WGA) (3),
– 网状分枝扩增(RAM) (4)。 - 随机引物法DNA标记。
不适用于PCR扩增。
酶活性在以下反应混合物中测试:10 mM Tris-HCl (pH 7.5), 10 mM MgCl2 , 100 µg/ml BSA, 0.75 mM活化鲑鱼精DNA, 0.2 mM各种dNTP, 0.4 MBq/ml [H3 ]-dTTP。
10 mM Tris-HCl (pH 7.5), 50 mM KCl, 1 mM DTT, 0.1 mM EDTA, 0.15% (v/v) Triton X-100, 50% (v/v) 甘油。
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- 作者
- 内容
- 询问日期
文献和实验), where we have overcome both the size limitations and the restrictions of the cloning/subcloning procedures used in the Red/ET recombineering system. We describe the transfer of a very large DNA fragment (~55 kb) from one BAC into another unrelated BAC by the means
Radiolabeling of DNA with the Klenow Fragment of DNA Polymerase
The Klenow fragment of DNA polymerase I is a proteolytic fragment obtained by the treatment of DNA polymerase I with subtilisin. The fragment still has the polymerase and 3′–5′ exonuclease activity, but lacks the 5′–3′ exonuclease activity
Materials: TE solution 10 mM Tris (pH to 7.5) 1 mM EDTA (pH to 8.0 to dissolve) Frozen agarose gel piece containing the desired DNA fragment Supplies:
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