Dideoxy Sequencing Reactions Using Klenow Fragment DNA Polymerase 1
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Historically, DNA sequencing by primed synthesis and chain terminators has been performed using the Klenow fragment of DNA polymerase 1(1 . This enzyme was derived from E. coli . DNA Pol I which, in addition to polymerase activity, has both 3′–5′ and 5′–3′ exonuclease activities.
