Preparation of Genomic DNA from Bacteria- using Phase Lock GelTM
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Materials: see Solutions for Recipes
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TE buffer |
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10% (w/v) sodium dodecyl sulfate (SDS ) |
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20 mg/ml proteinase K |
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phenol\chloroform (50:50) |
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isopropanol |
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70% ethanol |
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3M sodium acetate pH 5.2 |
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Phase Lock GelTM (Eppendorf-Brinkmann) (http://www.eppendorf.com/phaselockgel/en/phase_1.html )
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