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文献和实验using the AllPrep DNA/RNA 96 Kit. Real-time RT-PCR analysis of the PGK1 transcript was performed using a primer-probe set that could amplify and detect both mRNA and genomic DNA sequences. The flat amplification plot for the no RT control (-RT) indicates
down in microcentrifuge. 5) Set up program for a routine PCR reactions: Initial Denaturation 94-95°C for 1-5 min 25-40 cycles 94-95°C for 30 sec 45-70°C for 10-30 sec 72
Quantitative measurements of serum hepatitis B virus (HBV) DNA are useful for tailoring of treatment schedules and the monitoring of HBV replication during therapy. We developed a novel fluorescence-based quantitative real-time PCR
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