- ￥39860
- QIGEN
- 德国
- 9017596
- 2025年11月07日
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文献和实验down 5',5K,4℃ **wash means complete resuspention to remove all salt 6.Invert tube to drain SN ~5sec (soft plt).with p200,resuspend cells in remaining SN & transfer to eppendorf.Wash SS34 tube with another 100μl 10% glycerol and pool with cells.Vf ~300-500μl
Agrobacterium growth and transformation
. Set the resistance to 400 Ohm on the Pulse Controller Unit (note that this is higher than for E. coli, which is usually set for ~200 Ohm). 5. Place the cuvette in the cuvette holder, slide down to engage the electrodes and push both buttons
Agrobacterium growth and transformation (农杆菌培养及转化)
holder to the gene pulser. For cuvettes with a 2mm gap size, adjust the Gene Pulser unit "Set Volts"setting to 2.5 kV and the "CAP" setting to 25 uFD. Set the resistance to 400 Ohm on the Pulse Controller Unit (note that this is higher than for E. coli
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