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文献和实验24 µl H2O and 1 µl RNase inhibitor, 20 U/µl, to RNA pellet. Resuspend the RNA pellet with gentle tapping. Quick spin. Aliquot 12 µl into 2 tubes for the (+) and (-) RT reactions. Prepare additional control tubes using the following in place
Light-Controlled Gene Switches in Mammalian Cells
can be repeated in both directions. The iPEP acts as dominant-negative inhibitor targeting c-Jun and c-Fos of the transcription factor activator protein-1 (AP-1). Light-activated peptides exhibited much stronger inhibition of AP-1:DNA complexes and interference
The Regulation of Tumor Suppressor Genes by Oncogenes
Tumor suppressor genes (TSGs) and oncogenes represent the ying and the yang of cell growth, differentiation, and survival control. TSGs such as p53, the retinoblastoma (RB) gene product, and the cyclin kinase inhibitor (CKI) proteins p21 Cip
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