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文献和实验Hints and precautions for the care, feeding and breeding of Neurospora
, above 30°C. We routinely make crosses on SC slants in 18 X 150 mm tubes, using foam or cotton plugs. Push-on metal or plastic caps should be avoided with cross tubes because they result in more rapid water loss and are more prone to contamination
, the majority of which participate in the host immune response [1] including immunoglobulin (Ig) gene rearrangements [2,3] . Various types of stimuli (cytokines, CD40 ligation, TLR ligation, stress factors, microbial pathogens, etc.) activate and phosphorylate
) system, a microbial adaptive immune system, has transformedthe fi eld because of its easiness to design, rapidity to implement,low-cost, and scalability in eukaryotic cells. The CRISPR locus in microbes typically consists of a set ofnoncoding RNA elements
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