天津生物芯片通过基于第三代测序技术 PacBio RS II 的全基因组测序,可以快速低成本地组装出细菌的完成图,预测重要基因和蛋白以及了解其功能和机制。完成图在研究病原菌的致病性与疾病的预防和治疗方面,鉴定致病相关基因、开发和研究药物,疫苗、开发新型抗生素等方面具有非常重要的应用。同时它在研究细菌进化,生理和生态多样性,种内进化关系也有其他技术所不具备的优势。细菌 de novo 测序已取代传统方法成为研究细菌遗传机制,锁定关键功能基因的重要工具。
技术路线
参考文献 Koren, Sergey, et al. "Reducing assembly complexity of microbial genomes with single-molecule sequencing." arXiv preprint arXiv:1304.3752 (2013).
Bashir, Ali, et al. "A hybrid approach for the automated finishing of bacterial genomes." Nature biotechnology (2012).
Rasko, David A., et al. "Origins of the E. coli strain causing an outbreak of hemolytic–uremic syndrome in Germany." New England Journal of Medicine 365.8 (2011): 709-717.
Murray, Iain A., et al. "The methylomes of six bacteria." Nucleic acids research 40.22 (2012): 11450-11462.
Clark, Tyson A., et al. "Characterization of DNA methyltransferase specificities using single-molecule, real-time DNA sequencing." Nucleic Acids Research 40.4 (2012): e29-e29.