- 询价
- StressMarq
- 加拿大
- SPR-104
- 2025年11月23日
企业认证
万千商家帮你免费找货
0 人在求购买到急需产品
- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
-20ºC
- 保质期：
一年
- 英文名：
HSP60 Protein
- 库存：
大量
- 供应商：
StressMarq
- 规格：
50 µg/100 µg/100 µg x2
| 规格： | 50 µg | 产品价格： | 询价 |
|---|---|---|---|
| 规格： | 100 µg | 产品价格： | 询价 |
| 规格： | 100 µg x2 | 产品价格： | 询价 |
| 产品名称 | HSP60 蛋白 |
| 产品描述 |
活性重组人HSP60蛋白 |
| 应用范围 | WB, SDS-PAGE, ATPase Activity Assay, Functional Assay, ELISA |
| 浓度 | 各批次不同，请详见说明书 |
| 标记物 | His 标签 |
| 性质 | 重组 |
| 来源物种 | 人 |
| 表达系统 | 大肠杆菌 (E. coli) |
| 纯度 | >90% |
| Full Biological Activity | ATPase 活性 |
产品特性
| 储存缓冲液 | 20mM 磷酸缓冲液, 150mM NaCl , 10% 甘油 |
| 储存温度 | -20ºC |
| 运输温度 | 蓝冰或4℃ |
| 纯化方式 | 亲和纯化的 |
| Protein Size | 分子量约为60kD |
| 引用该产品 | Human Recombinant HSP60 Protein (StressMarq Biosciences Inc., Victoria BC CANADA, Catalog # SPR-104) |
| 分析证书 | 该蛋白已经通过SDS-PAGE检测证明纯度大于90%. 此蛋白出厂时的ATP酶活性为每小时从每微克蛋白质分离出3.6μM 磷酸根 (使用孔雀绿磷酸检测, 200μl反应液, 20ul的1mM ATP, 37°C, pH7.5 ). |
| 其他相关信息 | 具有ATP酶活性 |
生物学特性
| 别名 | 60kDa chaperonin Protein, cb863 Protein, CPN60 Protein, GROEL Protein, GroEL Homolog Protein, HLD4 Protein, Hsp65 Protein, HSPD1 Protein, HuCHA60 Protein, SPG13 Protein |
| 研究领域 | 伴侣蛋白, 标签和细胞标记物, 热休克, 癌症, 细胞信号传导, 细胞器标记物, 蛋白质运输 |
| 细胞定位 | 细胞核, 细胞质, 黑素体 |
| Accession Number | BC003030 |
| GeneID | 3329 |
| Swiss Prot | P10809 |
| 科研背景 | In both prokaryotic and eukaryotic cells, the misfolding and aggregation of proteins during biogenesis and under conditions of cellular stress are prevented by molecular chaperones. Members of the HSP60 family of heat shock proteins are some of the best characterized chaperones. HSP60, also known as Cpn60 or GroEl, is an abundant protein synthesized constitutively in the cell that is induced to a higher concentration after brief cell shock. It is present in many species and exhibits a remarkable sequence homology among various counterparts in bacteria, plants, and mammals with more than half of the residues identical between bacterial and mammalian HSP60 (1-3). Whereas mammalian HSP60 is localized within the mitochondria, plant HSP60, or otherwise known as Rubisco-binding protein, is located in plant chloroplasts. It has been indicated that these proteins carry out a very important biological function due to the fact that HSP60 is present in so many different species. The common characteristics of the HSP60s from the divergent species are i) high abundance, ii) induction with environmental stress such as heat shock, iii) homo-oligomeric structures of either 7 or 14 subunits which reversibly dissociate in the presence of Mg2+ and ATP, iv) ATPase activity and v) a role in folding and assembly of oligomeric protein structures (4). These similarities are supported by recent studies where the single-ring human mitochondrial homolog, HSP60 with its co-chaperonin, HSP10 were expressed in a E. coli strain, engineered so that the groE operon is under strict regulatory control. This study has demonstrated that expression of HSP60-HSP10 was able to carry out all essential in vivo functions of GroEL and its co-chaperonin, GroES (5). Another important function of HSP60 and HSP10 is their protective functions against infection and cellular stress. HSP60 has however been linked to a number of autoimmune diseases, as well as Alzheimer's, coronary artery diseases, MS, and diabetes (6-9). |
| 参考资料 | 1. Hartl F.U. (1996) Nature. 381: 571-579. 2. Bukau B. and Horwich A.L. (1998) Cell. 92: 351-366. 3.Hartl F.U. and Hayer-Hartl M. (2002) Science. 295: 1852-1858. 4. Jindal S., et al. (1989) Molecular and Cellular Biol. 9: 2279-2283. 5. La Verda D., et al (1999) Infect Dis. Obstet. Gynecol. 7: 64-71. 6. Itoh H., et al. (2002) Eur. J. Biochem. 269: 5931-5938. 7.Gupta S. and Knowlton A.A. J. Cell Mol Med. 9: 51-58. 8. Deocaris C.C. et al. (2006) Cell Stress Chaperones. 11: 116-128. 9. Lai H.C., et al. (2007) Am. J. Physiol. Endocrinol. Metab. 292: E292-E297. |
产品图片
60kDa Hsp60 蛋白 SDS-PAGE (SPR-104).
风险提示：丁香通仅作为第三方平台，为商家信息发布提供平台空间。用户咨询产品时请注意保护个人信息及财产安全，合理判断，谨慎选购商品，商家和用户对交易行为负责。对于医疗器械类产品，请先查证核实企业经营资质和医疗器械产品注册证情况。
文献和实验[1] Dieudé M, Correa J A, Neville C, et al. Association of autoantibodies to heat‐shock protein 60 with arterial vascular events in patients with antiphospholipid antibodies[J]. Arthritis & Rheumatism, 2011, 63(8): 2416-2424.
[2] Werner C, Stangl S, Salvermoser L, et al. Hsp70 in liquid biopsies—A tumor-specific biomarker for detection and response monitoring in cancer[J]. Cancers, 2021, 13(15): 3706.
[3] Hu F, Guo Q, Wei M, et al. Chlorogenic acid alleviates acetaminophen-induced liver injury in mice via regulating Nrf2-mediated HSP60-initiated liver inflammation[J]. European Journal of Pharmacology, 2020, 883: 173286.
植物源重组人胰岛素生长因子融合蛋白（OsrIGF-1），胰岛素在无血清细胞培养中的最佳替代品。 IGFs家族由两种低分子多肽(IGF-Ⅰ、IGF-Ⅱ)、两类特异性受体及六种结合蛋白组成。IGF-Ⅰ是一个有70个氨基酸的单链碱性蛋白，分子量7649Da，耐热。IGFs的生物学功能不只局限于有丝分裂刺激作用，它们也能诱导分化或促进分化功能的表达。全新推出的植物源重组人类胰岛素生长因子-1融合蛋白（OsrIGF-1）在IGF-1的N末端融合了人血清白蛋白（HSA），HSA作为蛋白质保护剂增加了IGF
Isolation and Application of Bioactive Wnt Proteins Methods in Molecular Biology v468. chapter 2 Abstract Wnt proteins and their signaling cascades are involved in a wide variety of developmental processes, andderegulation
上海西唐生物科技有限公司 021-55229872, 65333639 www.westang.com 人热休克蛋白60 ( HSP60 )ELISA 试剂盒 原理 本实验采用双抗体夹心 ABC-ELISA 法。用抗人 HSP60 单抗包被于酶标板上，标准品和样品中的 HSP60与单抗结合，加入生物素化的抗人 HSP60 ，形成免疫复合物连接在板上，辣根
技术资料暂无技术资料 索取技术资料
