- 询价
- StressMarq
- 加拿大
- SPR-101
- 2026年01月12日
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- 详细信息
- 询价记录
- 文献和实验
- 技术资料
- 保存条件：
-20ºC
- 保质期：
一年
- 英文名：
HSP90 alpha Protein
- 库存：
大量
- 供应商：
StressMarq
- 规格：
50 µg/100 µg/100 µg x2
| 规格： | 50 µg | 产品价格： | 询价 |
|---|---|---|---|
| 规格： | 100 µg | 产品价格： | 询价 |
| 规格： | 100 µg x2 | 产品价格： | 询价 |
| 产品名称 | HSP90 alpha 蛋白 |
| 产品描述 |
重组人HSP90 alpha蛋白 |
| 应用范围 | WB, SDS-PAGE, Atpase Activity Assay, SPR, DARTs Assay, Skin Blotting |
| 浓度 | 各批次不同，请详见说明书 |
| 标记物 | 无标签 |
| 性质 | 重组 |
| 来源物种 | 人 |
| 表达系统 | 大肠杆菌 (E. coli) |
| 纯度 | >90% |
产品特性
| 储存缓冲液 | 50mM Tris/HCl pH 7.5, 5mM Bme, 0.3M NaCl, 10% 甘油 |
| 储存温度 | -20ºC |
| 运输温度 | 蓝冰或4℃ |
| 纯化方式 | 亲和纯化的 |
| Protein Size | 分子量约为90kD |
| 引用该产品 | Human Recombinant HSP90 alpha Protein (StressMarq Biosciences Inc., Victoria BC CANADA, Catalog # SPR-101) |
| 分析证书 | 该蛋白已经通过SDS-PAGE检测证明纯度大于90%. |
生物学特性
| 别名 | Hsp86 Protein, Hsp89A Protein, Hsp90A Protein, Hsp90AA1 Protein, HspC1 Protein, HspCA Protein, HspCAL3 Protein |
| 研究领域 | 伴侣蛋白, 热休克, 癌症, 细胞信号传导, 蛋白质运输, 肿瘤标记物 |
| 细胞定位 | 细胞质, 黑素体 |
| Accession Number | NP_001017963.2 |
| GeneID | 3320 |
| Swiss Prot | P07900 |
| 科研背景 | HSP90 is a highly conserved and essential stress protein that is expressed in all eukaryotic cells. From a functional perspective, HSP90 participates in the folding, assembly, maturation, and stabilization of specific proteins as an integral component of a chaperone complex (1-4). Despite its label of being a heat-shock protein, HSP90 is one of the most highly expressed proteins in unstressed cells (1–2% of cytosolic protein). It carries out a number of housekeeping functions – including controlling the activity, turnover, and trafficking of a variety of proteins. Most of the HSP90-regulated proteins that have been discovered to date are involved in cell signaling (5-6). The number of proteins now know to interact with HSP90 is about 100. Target proteins include the kinases v-Src, Wee1, and c-Raf, transcriptional regulators such as p53 and steroid receptors, and the polymerases of the hepatitis B virus and telomerase.5. When bound to ATP, HSP90 interacts with co-chaperones Cdc37, p23, and an assortment of immunophilin-like proteins, forming a complex that stabilizes and protects target proteins from proteasomal degradation. In most cases, HSP90-interacting proteins have been shown to co-precipitate with HSP90 when carrying out immunoadsorption studies, and to exist in cytosolic heterocomplexes with it. In a number of cases, variations in HSP90 expression or HSP90 mutation has been shown to degrade signaling function via the protein or to impair a specific function of the protein (such as steroid binding, kinase activity) in vivo. Ansamycin antibiotics, such as geldanamycin and radicicol, inhibit HSP90 function (7). Looking for more information on HSP90? Visit our new HSP90 Scientific Resource Guide. |
| 参考资料 | 1. Arlander S.J.H., et al. (2003) J Biol Chem. 278: 52572-52577. 2. Pearl H., et al. (2001) Adv Protein Chem. 59:157-186. 3. Neckers L., et al. (2002) Trends Mol Med. 8:S55-S61. 4. Pratt W., Toft D. (2003) Exp Biol Med. 228:111-133. 5. Pratt W., Toft D. (1997) Endocr Rev. 18: 306–360. 6. Pratt W.B. (1998) Proc Soc Exptl Biol Med. 217: 420–434. 7. Whitesell L., et al. (1994) Proc Natl Acad Sci USA. 91: 8324– 8328. |
产品图片
人 HSP90 Alpha 蛋白SDS-Page (SPR-101). 泳道 1: 分子量梯 (MW). 泳道 2: 人HSP90 alpha 蛋白 (SPR-101).
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- 作者
- 内容
- 询问日期
文献和实验[1] Wang J, Grishin A V, Ford H R. Experimental anti-inflammatory drug semapimod inhibits TLR signaling by targeting the TLR chaperone gp96[J]. The Journal of Immunology, 2016, 196(12): 5130-5137.
[2] Bartolini M, Wainer I W, Bertucci C, et al. The rapid and direct determination of ATPase activity by ion exchange chromatography and the application to the activity of heat shock protein-90[J]. Journal of pharmaceutical and biomedical analysis, 2013, 73: 77-81.
植物源重组人胰岛素生长因子融合蛋白（OsrIGF-1），胰岛素在无血清细胞培养中的最佳替代品。 IGFs家族由两种低分子多肽(IGF-Ⅰ、IGF-Ⅱ)、两类特异性受体及六种结合蛋白组成。IGF-Ⅰ是一个有70个氨基酸的单链碱性蛋白，分子量7649Da，耐热。IGFs的生物学功能不只局限于有丝分裂刺激作用，它们也能诱导分化或促进分化功能的表达。全新推出的植物源重组人类胰岛素生长因子-1融合蛋白（OsrIGF-1）在IGF-1的N末端融合了人血清白蛋白（HSA），HSA作为蛋白质保护剂增加了IGF
【求助】矛盾的实验结果：IKb alpha和NFKB同时升高
吴佰霖 我的基因过表达，结合文献，推理应该是IKb alpha下降，而NFKB升高 （IKb可磷酸化NFKB，使后者降低） 但现在我的结果是两者同时升高，请问可能的原因有哪些呢？ 谢谢 woxingwosu 可否检测一下NFKB的磷酸化情况？ erik NFKB是检测核内的吗？检测p65还是p50/ 吴佰霖 检测总蛋白
Detecting HSP90 Phosphorylation
Heat-shock protein 90 (HSP90 ) is an essential molecular chaperone in eukaryotes. It is important for chaperoning proteins that are important determinants of multistep carcinogenesis. HSP90 ’s ATPase activity is associated with its chaperone
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