- 询价
- Cell Signaling Technology
- 3579
- USA
- 2026年05月28日
- western blot,免疫组化（IHC）,免疫荧光（IF）,流式细胞（Flow Cyt）
- 兔
- 人,驴,细菌,狗,马
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- 文献和实验
- 技术资料
- 库存：
大量
- 保存条件：
-20°c
- 供应商：
CST
- 抗原：
/
- 宿主：
兔
- 级别：
详见MSDS文件
- 抗原来源：
/
- 保质期：
详见说明书
- 抗体英文名：
Sox2 (D6D9) XP Rabbit mAb
- 是否单克隆：
1
- 适应物种：
人,驴,细菌,狗,马
- 应用范围：
western blot,免疫组化（IHC）,免疫荧光（IF）,流式细胞（Flow Cyt）
- 规格：
40 ul (4 western blots)/100 ul (10 western blots)/<a href="http://www.cellsignal.com/ddt/custom_reagents.html" target="_blank">carrier free & custom formulation / quantity</a> /40 ul (4 western blots)/100 ul (10 western blots)/<a href="http://www.cellsignal.com/ddt/custom_reagents.html" target="_blank">carrier free & custom formulation / quantity</a>
| 规格： | 产品价格： | ￥询价 | |
|---|---|---|---|
| 规格： | 40 ul (4 western blots) | 产品价格： | ￥请询价 |
| 规格： | 100 ul (10 western blots) | 产品价格： | ￥请询价 |
| 规格： | <a href="http://www.cellsignal.com/ddt/custom_reagents.html" target="_blank">carrier free & custom formulation / quantity</a> | 产品价格： | ￥请询价 |
| 规格： | 40 ul (4 western blots) | 产品价格： | ￥请询价 |
| 规格： | 100 ul (10 western blots) | 产品价格： | ￥请询价 |
| 规格： | <a href="http://www.cellsignal.com/ddt/custom_reagents.html" target="_blank">carrier free & custom formulation / quantity</a> | 产品价格： | ￥请询价 |
Product Pathways - Wnt / Hedgehog / Notch
Sox2 (D6D9) XP® Rabbit mAb #3579
PhosphoSitePlus ® protein, site, and accession data: SOX2
| Applications | Reactivity | Sensitivity | MW (kDa) | Isotype |
|---|---|---|---|---|
| W IHC-P IF-IC F | H (Mk) (B) (Dg) (Hr) | Endogenous | 35 | Rabbit IgG |
Applications Key: W=Western Blotting IHC-P=Immunohistochemistry (Paraffin) IF-IC=Immunofluorescence (Immunocytochemistry) F=Flow Cytometry
Reactivity Key: H=Human Mk=Monkey B=Bovine Dg=Dog Hr=Horse
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.
Protocols
- 3579:
- Flow , IHC / Paraffin , Immunofluorescence , Immunoprecipitation , Western Blotting
Specificity / Sensitivity
Sox2 (D6D9) XP® Rabbit mAb detects endogenous levels of Sox2 protein.
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to amino acids surrounding Gly179 of human Sox2.
Western Blotting
Western blot analysis of extracts from NTERA2 and NCCIT cells using Sox2 (D6D9) XP® Rabbit mAb.
IHC-P (paraffin)
Immunohistochemical analysis of paraffin-embedded human squamous cell carcinoma of the esophagus using Sox2 (D6D9) XP (TM) Rabbit mAb.
IHC-P (paraffin)
Immunohistochemical anaylsis of paraffin-embedded NTERA2 cells (positive) (left) and HeLa cells (negative) (right) using Sox2 (D6D9) XP® Rabbit mAb.
Flow Cytometry
Flow cytometric analysis of HeLa cells (blue) and NTERA2 cells (green) using Sox2 (D6D9) XP® Rabbit mAb.
IF-IC
Confocal immunofluorescent analysis of NTERA2 (left) and HeLa (right) cells using Sox2 (D6D9) XP® Rabbit mAb (green). Actin filaments have been labeled with DY-554 phalloidin (red).
Background
Embryonic stem cells are derived from the inner cell mass of the blastocyst and are unique in their pluripotent capacity and potential for self-renewal. Sox2 is one of a set of transcription factors that are crucial for the maintenance of pluripotency (1). Sox2, Oct-4, and Nanog cooperate in this network (1-3), and siRNA knockdown of either Sox2 or Oct-4 results in loss of pluripotency (4,5). Chromatin immunoprecipitation experiments have shown that Sox2 and Oct-4 bind to thousands of gene regulatory sites, highlighting the importance of these transcription factors in early embryonic development (6,7). It has recently been shown that Sox2 is amplified in lung and esophageal squamous cell tumors (8).
- Nichols, J. et al. (1998) Cell 95, 379-391.
- Avilion, A.A. et al. (2003) Genes Dev. 17, 126-140.
- Rodda, D.J. et al. (2005) J. Biol. Chem. 280, 24731-24737.
- Matin, M.M. et al. (2004) Stem Cells 22, 659-668.
- Niwa, H. et al. (2000) Nat. Genet. 24, 372-376.
- Boyer, L.A. et al. (2005) Cell 122, 947-956.
- Loh, Y.H. et al. (2006) Nat. Genet. 38, 431-440.
- Bass, A.J. et al. (2009) Nat Genet 41, 1238-42.
Application References
Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know !
Companion Products
- 5024 Sox2 (D6D9) XP® Rabbit mAb (ChIP Formulated)
- 2748 Sox2 Antibody
- 3978 LIN28A (A177) Antibody
- 3580 Nanog Antibody
- 2840 Oct-4A (C30A3) Rabbit mAb
- 2750 Oct-4 Antibody
- 7071 Phototope® -HRP Western Blot Detection System, Anti-rabbit IgG, HRP-linked Antibody
- 7074 Anti-rabbit IgG, HRP-linked Antibody
- 7720 Prestained Protein Marker, Broad Range (Premixed Format)
- 7727 Biotinylated Protein Ladder Detection Pack
- 7003 20X LumiGLO® Reagent and 20X Peroxide
- 8114 SignalStain® Boost IHC Detection Reagent (HRP, Rabbit)
- 8112 SignalStain® Antibody Diluent
- 5425 Normal Goat Serum
- 9997 Tris Buffered Saline with Tween 20 (TBST-10X)
For Research Use Only. Not For Use In Diagnostic Procedures.
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文献和实验稀释液的效果更好。4. 匹配的二抗和显色系统——「配一配和滴一滴」使用多聚 HRP（Poly-HRP）的二抗，即在二抗上连接大量 HRP，以充分放大信号；同时，配合优质的色原产品，提升灵敏度，增强信号。注：在人肺癌标本中使用 Sox2(D6D9) XP® RabbitmAb#3579 检测 Sox2 的表达，使用基于多聚-HRP 的 SignalStain® BoostIHCDetectionReagent 的检测灵敏度更高。注：DAB 色原比较：在人乳腺癌组织中使用 p-Stat3(Tyr705)(D
性生物素所致的假阳性染色，其检测过程省去一个步骤为检测过程节省了时间，并且其与所有基于过氧化物酶的底物相容。抗体：Sox2 (D6D9) XP. Rabbit mAb #3579样本：石蜡包埋的人类肺部肿瘤检测：基于生物素的检测（左）或基于聚合物的检测（SignalStain. Boost IHC Detection Reagent #8114;（右）色原二氨基联苯胺 (DAB) 底物是基于过氧化物酶检测系统中最常用的色原之一。DAB 与 HRP 反应以在抗体结合处形成棕色沉淀物。在实验中建议始终
组织化学分析。（图 C）使用Anti-Erk1/2 Mouse mAb 鼠单抗进行目的蛋白检测；(图 D) 使用p44/42 MAPK （Erk1/2）Rabbit mAb 兔单抗进行目的蛋白检测。 图 7 免疫组化实验检测Akt表达 注：使用 Anti-Akt (pan) Rabbit mAb 对正常小鼠肝组织进行免疫组织化学分析。（图 E）使用免疫组化试剂盒 M&R HRP/DAB Detection IHC Kit，抗体1:100 稀释；(图F)使用另一种试剂盒，抗体 1:100 稀释。
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