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- 详细信息
- 文献和实验
- 技术资料
- 免疫原:
A synthesized peptide derived from human sox2 around the phosphorylation site of Ser249/250
- 形态:
liquid
- 保存条件:
Store at -20˚C
- 克隆性:
Polyclonal
- 适应物种:
Hu, Ms, Rt
- 宿主:
Rabbit
- 应用范围:
WB
- 浓度:
1mg/ml
- 抗体英文名:
sox2 (Phospho-Ser249/250) Antibody
clonality:Polyclonal
specificity:Phospho-sox2 (Phospho-Ser249/250) Antibody detects endogenous levels of sox2 only when phosphorylated at Ser249/250
other_names:ANOP3; cb236; Delta EF2a; lcc; MCOPS3; MGC148683; MGC2413; RGD1565646; Sex determining region Y box 2; SOX 2; Sox2; SOX2_HUMAN; SRY (sex determining region Y) box 2; SRY box containing gene 2; SRY related HMG box 2; SRY related HMG box gene 2; SRY-box 2; Transcription factor SOX 2; Transcription factor SOX-2; ysb; accession_no:Swiss-Prot#:P48431
NCBI Gene ID:6657
产品详细信息请点击:sox2 (Phospho-Ser249/250) Antibody
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文献和实验secondary antibody review -- data from 99 publications
IgG Alexa Fluor 488 1:250 Molecular Probes 10 goat Cy3 immunocytochemistry detect NMDA receptors in HEK293 cells Jackson ImmunoResearch Laboratories 10 FITC immunocytochemistry 1:100 detect phospho-CREB in human colonic
TBS 和 5% NGS 中封闭样品。市售的含有酪蛋白的封闭溶液与磷酸化的一抗结合后易减弱信号;因此,我们建议不使用含有酪蛋白的封闭剂进行磷酸化特异性抗体的检测。以上针对封闭步骤所提建议均是在以 SignalStain. Boost IHC Detection Reagent 作为检测试剂的情况下提出的。如果选择使用其他检测试剂,我们则建议使用与二抗来源相同的血清进行封闭。抗体:Phospho–Histone H2A.X (Ser139) (20E3) Rabbit mAb #9718样本:石蜡
antibody dilution buffer: TBS-T supplemented with2% (w/v) BSA. 6. Antibodies: Phospho-b-catenin Ser33/Ser37/Thr41, phospho-glycogen synthase Ser641, and glycogen synthase (CellSignaling Technologies, Beverly, MA); b-catenin and GSK-3b(BD PharMingen, San
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